University of Tampere, Institute of Biomedical Technology and BioMediTech, Biokatu 6, 33520 Tampere, Finland.
Phys Chem Chem Phys. 2014 Apr 14;16(14):6342-57. doi: 10.1039/c3cp54884h. Epub 2014 Jan 28.
The dynamic regulation of cell-matrix adhesion is essential for tissue homeostasis and architecture, and thus numerous pathologies are linked to altered cell-extracellular matrix (ECM) interaction and ECM scaffold. The molecular machinery involved in cell-matrix adhesion is complex and involves both sensory and matrix-remodelling functions. In this review, we focus on how protein conformation controls the organization and dynamics of cell-matrix adhesion. The conformational changes in various adhesion machinery components are described, including examples from ECM as well as cytoplasmic proteins. The discussed mechanisms involved in the regulation of protein conformation include mechanical stress, post-translational modifications and allosteric ligand-binding. We emphasize the potential role of intrinsically disordered protein regions in these processes and discuss the role of protein networks and co-operative protein interactions in the formation and consolidation of cell-matrix adhesion and extracellular scaffolds.
细胞-基质黏附的动态调控对于组织稳态和结构至关重要，因此许多病理学与细胞-细胞外基质（ECM）相互作用和 ECM 支架的改变有关。参与细胞-基质黏附的分子机制很复杂，涉及感知和基质重塑功能。在这篇综述中，我们重点关注蛋白质构象如何控制细胞-基质黏附的组织和动态。描述了各种黏附机械组件的构象变化，包括 ECM 以及细胞质蛋白的例子。讨论的涉及蛋白质构象调节的机制包括机械应激、翻译后修饰和变构配体结合。我们强调了在这些过程中固有无序蛋白区域的潜在作用，并讨论了蛋白质网络和协同蛋白质相互作用在细胞-基质黏附和细胞外支架形成和巩固中的作用。
