Su Duo, Zhou Tianhui, Wang Yan, Wang Linjie
Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
Int J Mol Sci. 2023 Sep 20;24(18):14330. doi: 10.3390/ijms241814330.
Cold exposure influences liver metabolism, thereby affecting energy homeostasis. However, the gene regulatory network of the liver after cold exposure remains poorly understood. In this study, we found that 24 h cold exposure (COLD, 6 °C) increased plasma glucose (GLU) levels, while reducing plasma non-esterified fatty acid (NEFA) and triglyceride (TG) levels compared to the room temperature (RT, 25 °C) group. Cold exposure increased hepatic glycogen content and decreased hepatic lipid content in the livers of newborn goats. We conducted RNA-seq analysis on the livers of newborn goats in both the RT and cold exposure groups. A total of 1600 genes were identified as differentially expressed genes (DEGs), of which 555 genes were up-regulated and 1045 genes were down-regulated in the cold exposure group compared with the RT group. Cold exposure increased the expression of genes involved in glycolysis, glycogen synthesis, and fatty acid degradation pathways. These results can provide a reference for hepatic lipid and glycogen metabolism in newborn goats after cold exposure.
冷暴露会影响肝脏代谢，从而影响能量平衡。然而，冷暴露后肝脏的基因调控网络仍知之甚少。在本研究中，我们发现与室温（RT，25°C）组相比，24小时冷暴露（COLD，6°C）会增加血浆葡萄糖（GLU）水平，同时降低血浆非酯化脂肪酸（NEFA）和甘油三酯（TG）水平。冷暴露增加了新生山羊肝脏中的肝糖原含量，并降低了肝脏脂质含量。我们对RT组和冷暴露组的新生山羊肝脏进行了RNA测序分析。共鉴定出1600个基因作为差异表达基因（DEG），与RT组相比，冷暴露组中有555个基因上调，1045个基因下调。冷暴露增加了参与糖酵解、糖原合成和脂肪酸降解途径的基因表达。这些结果可为冷暴露后新生山羊的肝脏脂质和糖原代谢提供参考。
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