Wang Hong-Dan, Ruan Ji-Gang, Shen Chun-Mei, Zhang Yu-Dang, Liu Wen-Juan, Meng Hao-Tian, Yang Xin-Yuan, Yan Jiang-Wei, Liao Shi-Xiu, Fan Shuan-Liang, Jia Xiao-di, Wei Xing
Medical Genetic Institute of Henan Province, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, 450003, People's Republic of China.
Mol Biol Rep. 2014;41(4):2371-5. doi: 10.1007/s11033-014-3091-z. Epub 2014 Jan 28.
We obtained the allelic frequencies and forensic efficiency data for eight mini short tandem repeat loci including Penta E, D12S391, D6S1043, D2S1338, D19S433, CSF1PO, Penta D and D19S253 loci from a sample of 128 unrelated Uyghur individuals from China. The amplification products of the eight STR loci are <240 bp in size. A total of 94 alleles were observed and the corresponding allelic frequencies ranged from 0.0039 to 0.3438 in the present study. Observed genotype distributions for each locus do not show deviations from Hardy-Weinberg equilibrium expectations. The combined power of discrimination, combined power of exclusion and combined matching probability of the eight STR loci equaled to 0.999999999963373, 0.9997770 and 3.6627 × 10(-11), respectively. Because of the small fragment length of PCR products and the high degree of polymorphisms, the eight STR loci are highly beneficial for the forensic analysis of degraded DNA samples which are commonly observed in forensic cases. The STR data of the Uyghur group were compared with the previously published population STR data of other groups from different ethnic or areas, and significant differences were observed among these groups at some loci.
我们从128名来自中国的维吾尔族无关个体样本中获取了包括五核苷酸重复序列E（Penta E）、D12S391、D6S1043、D2S1338、D19S433、CSF1PO、五核苷酸重复序列D（Penta D）和D19S253位点在内的8个微型短串联重复序列位点的等位基因频率和法医学效率数据。这8个短串联重复序列位点的扩增产物大小均小于240 bp。本研究共观察到94个等位基因，相应的等位基因频率范围为0.0039至0.3438。每个位点观察到的基因型分布均未显示出偏离哈迪-温伯格平衡预期的情况。这8个短串联重复序列位点的联合鉴别力、联合排除力和联合匹配概率分别为0.999999999963373、0.9997770和3.6627×10⁻¹¹。由于聚合酶链反应产物片段长度较短且多态性程度高，这8个短串联重复序列位点对于法医案件中常见的降解DNA样本的法医分析极为有利。将维吾尔族群体的短串联重复序列数据与先前发表的来自不同种族或地区的其他群体的群体短串联重复序列数据进行比较，发现这些群体在某些位点存在显著差异。
