Hadian Javad, Bigdeloo Mahdi, Nazeri Vahideh, Khadivi-Khub Abdollah
Department of Agriculture, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, G.C., Evin, 1483963113, Tehran, Iran.
Mol Biol Rep. 2014 May;41(5):3201-10. doi: 10.1007/s11033-014-3180-z. Epub 2014 Jan 29.
Thymus caramanicus is an endemic species grown in Iran with interesting pharmacological and biological properties. In the present work, essential oil compositions and inter-simple sequences repeat (ISSR) markers were used to estimate the relationships among and within seven populations of T. caramanicus, belonging to three provinces in Iran. The studied individuals were distinguished on the basis of ISSR markers and constituents of essential oil. A total of 127 band positions were produced by 12 ISSR primers, of which 105 were found polymorphic with 82.68% polymorphism. Genetic similarity values among individuals ranged between 0.15 and 0.82 which was indicative of a high level of genetic variation. On the basis of their genetic similarities, ISSR analysis allowed to group the samples into two main clusters. One of these included populations originated from Kerman and Isfahan provinces, and the other cluster consists of populations from Semnan province. Chemical compounds of essential oils were found variable in the various individuals and all samples were principally composed of phenolic constituents (carvacrol and/or thymol). As a consequence, the plants were classified into two major chemotypes including carvacrol and thymol/carvacrol. A relationship between genetic and chemical variability and geographic distribution has been observed in studied populations of T. caramanicus.
卡拉曼胸腺是生长在伊朗的一种特有物种，具有有趣的药理和生物学特性。在本研究中，利用精油成分和简单序列重复区间（ISSR）标记来评估伊朗三个省份的七个卡拉曼胸腺种群之间以及种群内部的关系。根据ISSR标记和精油成分对所研究的个体进行区分。12条ISSR引物共产生了127个条带位置，其中105个具有多态性，多态性比例为82.68%。个体间的遗传相似性值在0.15至0.82之间，这表明存在高水平的遗传变异。基于遗传相似性，ISSR分析将样本分为两个主要聚类。其中一个聚类包括来自克尔曼省和伊斯法罕省的种群，另一个聚类由来自塞姆南省的种群组成。精油的化学成分在不同个体中存在差异，所有样本主要由酚类成分（香芹酚和/或百里香酚）组成。因此，这些植物被分为两种主要的化学型，包括香芹酚型和百里香酚/香芹酚型。在卡拉曼胸腺的研究种群中观察到了遗传和化学变异性与地理分布之间的关系。
