Immunology Research Centre, BuAli Research Institute, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pathology, Imam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Basic Med Sci. 2013 Mar;16(3):242-6.
OBJECTIVE(S): Non-Hodgkin's lymphoma (NHL) is a lymphoproliferative malignancy in which cells undergo microscopic changes with unknown etiology, and risk factors such as age, sex, genetic and environmental factors are involved. The relationship between the presence of infectious agents and the development of lymphoproliferative diseases has been an interesting research topic. HTLV-I (Human T Cell Lymphotropic Virus Type-1) predisposes the infected individulas to opportunistic neoplasms and lymphoid malignancies. HCV (Hepatitis C Virus) the etiologic agent of hepatitis C, is hepatotropic, and long-term infection with HCV can continuously stimulate and expand lymphocyte clones, resulting in further transformation and finally aggressive malignancies.
54 tissue samples diagnosed with NHL were selected to be studied for the presence of HTLV-I and HCV viruses. DNA and RNA were extracted from samples, cDNA was synthesized and using specific primers presence of HTLV-I and HCV viruses were investigated by PCR and nested RT-PCR methods.
In 10 out of 54 (18.8%) samples (7 men and 3 women), HTLV-I was present, and 4 out of 54 (7.4%) samples (3 men and one woman) were positive for HCV.
Based on our results, it is recommended that in patients with NHL, infection with HTLV-I and HCV viruses need to be screened.
非霍奇金淋巴瘤（NHL）是一种淋巴增生性恶性肿瘤，其中细胞发生微观变化，病因不明，涉及年龄、性别、遗传和环境等危险因素。感染因子与淋巴增生性疾病发展之间的关系一直是一个有趣的研究课题。HTLV-I（人类 T 细胞淋巴瘤病毒 1 型）使感染个体易患机会性肿瘤和淋巴恶性肿瘤。HCV（丙型肝炎病毒）是丙型肝炎的病原体，具有嗜肝性，HCV 的长期感染可不断刺激和扩增淋巴细胞克隆，导致进一步转化，最终发展为侵袭性恶性肿瘤。
选择 54 份经诊断为 NHL 的组织样本进行 HTLV-I 和 HCV 病毒检测。从样本中提取 DNA 和 RNA，合成 cDNA，并使用特异性引物通过 PCR 和巢式 RT-PCR 方法检测 HTLV-I 和 HCV 病毒的存在。
在 54 个样本中的 10 个（18.8%）中存在 HTLV-I，在 54 个样本中的 4 个（7.4%）中存在 HCV。
根据我们的结果，建议对 NHL 患者进行 HTLV-I 和 HCV 病毒感染的筛查。
