Ribeiro Jucienne Salgado, Maciel José Vinicius Bolognesi, Knop Luégya Amorin Henriques, Machado Maria Ângela Naval, Grégio Ana Maria Trindade, Camargo Elisa Souza
Dentistry Graduate Program/Orthodontics, PUCPR - Catholic University of Paraná, CuritibaPR, Brazil.
Department of Orthodontics, UFRJ - Federal University of Rio de Janeiro, Rio de JaneiroRJ, Brazil.
Braz Dent J. 2013 Sep-Oct;24(5):503-7. doi: 10.1590/0103-6440201302286.
The aim of this study was to evaluate, by histological analysis, the effect of growth hormone (GH) on periodontal ligament and alveolar bone during experimental tooth movement in rats. Eighty male Wistar rats divided into control (C) and experimental (E) groups were examined after 3, 7, 14 and 21 days under controlled climate conditions. Orthodontic force (30 cN) was applied on the maxillary first molar by an orthodontic appliance. Group E received 0.1 IU/kg/day of GH and Group C received 0.5 mL/kg/day of saline. The samples were processed and evaluated under optical microscopy and polarized light microscopy. The Kruskal Wallis test was applied to compare the intergroup variables at 5% significance level. Group E presented a larger number of osteoclasts on the 3rd and 7th days and Howship lacunae on the 3 rd day, a smaller number of blood vessels and greater amount of mature collagen on the 3 rd and 7 th days than Group C (p<0.05). It was concluded that GH accelerated and intensified bone resorption and produced delay in immature collagen formation during experimental tooth movement.
本研究旨在通过组织学分析，评估生长激素（GH）对大鼠实验性牙齿移动过程中牙周膜和牙槽骨的影响。将80只雄性Wistar大鼠分为对照组（C）和实验组（E），在可控气候条件下饲养3、7、14和21天后进行检查。通过正畸矫治器对上颌第一磨牙施加正畸力（30 cN）。E组接受0.1 IU/kg/天的GH，C组接受0.5 mL/kg/天的生理盐水。对样本进行处理，并在光学显微镜和偏振光显微镜下进行评估。采用Kruskal Wallis检验，以5%的显著性水平比较组间变量。与C组相比，E组在第3天和第7天破骨细胞数量更多，在第3天出现Howship陷窝，在第3天和第7天血管数量更少，成熟胶原量更多（p<0.05）。得出的结论是，在实验性牙齿移动过程中，GH加速并强化了骨吸收，并导致未成熟胶原形成延迟。
