Shintcovsk Ricardo Lima, Knop Luégya, Tanaka Orlando Motohiro, Maruo Hiroshi
Dental Press J Orthod. 2014 Mar-Apr;19(2):96-107. doi: 10.1590/2176-9451.19.2.096-107.oar.
Nicotine is harmful to angiogenesis, osteogenesis and synthesis of collagen.
The aim of this study was to investigate the effect of nicotine on bone remodeling during orthodontic movement in rats.
Eighty male Wistar rats were randomly divided into three groups: Group C (control), group CM (with orthodontic movement) and group NM (nicotine with orthodontic movement) groups. The animals comprising groups C and CM received 0.9% saline solution while group NM received nicotine solution (2 mg/kg). A nickel-titanium closed-coil spring was used to induce tooth movement. The animals were euthanized and tissue specimens were histologically processed. Blood vessels, Howship's lacunae and osteoclast-like cells present in the tension and compression areas of periodontal ligaments were quantified. The extent of bone formation was evaluated under polarized light, to determine the percentage of immature/mature collagen.
It was observed lower blood vessel densities in the NM group in comparison to the CM group, three (p < 0.001) and seven (p < 0.05) days after force application. Osteoclast-like cells and Howship's lacunae in the NM group presented lower levels of expression, in comparison to the CM group, with significant differences on day 7 (p < 0.05 for both variables) and day 14 (p < 0.05 for osteoclast-like cells and p < 0.01 for Howship's lacunae). The percentage of immature collagen was increased in the NM group in comparison to the CM group, with a statistically significant difference on day 3 (p < 0.05), day 7 (p < 0.001), day 14 (p < 0.001) and day 21 (p < 0.001).
Nicotine affects bone remodeling during orthodontic movement, reducing angiogenesis, osteoclast-like cells and Howship's lacunae, thereby delaying the collagen maturation process in new bone matrix.
尼古丁对血管生成、骨生成和胶原蛋白合成有害。
本研究旨在探讨尼古丁对大鼠正畸移动过程中骨重塑的影响。
80只雄性Wistar大鼠随机分为三组：C组（对照组）、CM组（正畸移动组）和NM组（尼古丁加正畸移动组）。C组和CM组动物接受0.9%生理盐水，而NM组接受尼古丁溶液（2mg/kg）。使用镍钛闭合螺旋弹簧诱导牙齿移动。对动物实施安乐死后，对组织标本进行组织学处理。对牙周膜张力和压缩区域中的血管、豪氏陷窝和破骨细胞样细胞进行定量分析。在偏振光下评估骨形成程度，以确定未成熟/成熟胶原蛋白的百分比。
与CM组相比，在施加力后第3天（p<0.001）和第7天（p<0.05），观察到NM组的血管密度较低。与CM组相比，NM组中的破骨细胞样细胞和豪氏陷窝表达水平较低，在第7天（两个变量p<0.05）和第14天（破骨细胞样细胞p<0.05，豪氏陷窝p<0.01）有显著差异。与CM组相比，NM组中未成熟胶原蛋白的百分比增加，在第3天（p<0.05）、第7天（p<0.001）、第14天（p<0.001）和第21天（p<0.001）有统计学显著差异。
尼古丁影响正畸移动过程中的骨重塑，减少血管生成、破骨细胞样细胞和豪氏陷窝，从而延迟新骨基质中胶原蛋白的成熟过程。
