Fuentealba I C, Haywood S, Trafford J
Department of Veterinary Pathology, University of Liverpool, UK.
Liver. 1987 Oct;7(5):277-82. doi: 10.1111/j.1600-0676.1987.tb00356.x.
Histochemical methods have invariably shown a good correlation with copper analysis by absorption spectrophotometry in the identification of canine copper storage diseases. But, in Wilson's disease (WD) in humans no such correlation exists and similar discrepancies have also been observed in copper-loaded rats. This study attempts to quantify stainable copper in the livers of copper-loaded rats and relate this to the hepatic copper concentrations. Male rats fed a high copper diet (1500 ppm) for 16 weeks were killed at intervals. The livers were analysed for copper and graded according to stainable copper present in paraffin sections stained with rhodanine and rubeanic acid. Initially there was a good correlation between histochemically demonstrable copper and its total concentration, but subsequently, when high liver levels of the metal were present, copper staining was very variable. This unrealiability has similarities with WD, in which the higher hepatic concentrations of presymptomatic patients are difficult to detect by conventional copper stains. The variation in the binding of copper and its intracellular localisation suggested by these results may have considerable significance in the pathogenesis of copper storage diseases.
在犬类铜储存疾病的鉴定中，组织化学方法始终显示出与通过吸收分光光度法进行的铜分析具有良好的相关性。但是，在人类的威尔逊病（WD）中，不存在这种相关性，并且在铜负荷大鼠中也观察到了类似的差异。本研究试图量化铜负荷大鼠肝脏中可染色铜的含量，并将其与肝脏铜浓度相关联。给雄性大鼠喂食高铜饮食（1500 ppm）16周，每隔一段时间处死一批大鼠。对肝脏进行铜分析，并根据用罗丹宁和红氨酸染色的石蜡切片中存在的可染色铜进行分级。最初，组织化学可显示的铜与其总浓度之间存在良好的相关性，但随后，当肝脏中金属含量较高时，铜染色变化很大。这种不可靠性与WD相似，在WD中，常规铜染色难以检测到症状前患者较高的肝脏铜浓度。这些结果所提示的铜结合及其细胞内定位的变化可能在铜储存疾病的发病机制中具有重要意义。
