Timoneda Oriol, Núñez-Hernández Fernando, Balcells Ingrid, Muñoz Marta, Castelló Anna, Vera Gonzalo, Pérez Lester J, Egea Raquel, Mir Gisela, Córdoba Sarai, Rosell Rosa, Segalés Joaquim, Tomàs Anna, Sánchez Armand, Núñez José I
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona (UAB), Bellaterra, Barcelona, Spain ; Departament de Genètica Animal, Centre de Recerca en AgriGenòmica (CRAG), CSIC-IRTA-UAB-UB, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, Spain.
PLoS One. 2014 Jan 24;9(1):e86965. doi: 10.1371/journal.pone.0086965. eCollection 2014.
Porcine production is a primary market in the world economy. Controlling swine diseases in the farm is essential in order to achieve the sector necessities. Aujeszky's disease is a viral condition affecting pigs and is endemic in many countries of the world, causing important economic losses in the swine industry. microRNAs (miRNAs) are non-coding RNAs which modulates gene expression in animals, plants and viruses. With the aim of understanding miRNA roles during the Aujeszky's disease virus [ADV] (also known as suid herpesvirus type 1 [SuHV-1]) infection, the expression profiles of host and viral miRNAs were determined through deep sequencing in SuHV-1 infected porcine cell line (PK-15) and in an animal experimental SuHV-1 infection with virulent (NIA-3) and attenuated (Begonia) strains. In the in vivo approach miR-206, miR-133a, miR-133b and miR-378 presented differential expression between virus strains infection. In the in vitro approach, most miRNAs were down-regulated in infected groups. miR-92a and miR-92b-3p were up-regulated in Begonia infected samples. Functional analysis of all this over expressed miRNAs during the infection revealed their association in pathways related to viral infection processes and immune response. Furthermore, 8 viral miRNAs were detected by stem loop RT-qPCR in both in vitro and in vivo approaches, presenting a gene regulatory network affecting 59 viral genes. Most described viral miRNAs were related to Large Latency Transcript (LLT) and to viral transcription activators EP0 and IE180, and also to regulatory genes regarding their important roles in the host-pathogen interaction during viral infection.
生猪生产是世界经济中的一个主要市场。为了满足该行业的需求，控制猪场中的猪病至关重要。奥耶斯基氏病是一种影响猪的病毒性疾病，在世界许多国家都有流行，给养猪业造成了重大经济损失。微小RNA（miRNA）是一类非编码RNA，可调节动物、植物和病毒中的基因表达。为了了解miRNA在奥耶斯基氏病病毒[ADV]（也称为猪疱疹病毒1型[SuHV-1]）感染过程中的作用，通过深度测序确定了SuHV-1感染的猪细胞系（PK-15）以及用强毒株（NIA-3）和弱毒株（秋海棠）进行动物实验性SuHV-1感染时宿主和病毒miRNA的表达谱。在体内实验中，miR-206、miR-133a、miR-133b和miR-378在不同病毒株感染之间呈现差异表达。在体外实验中，大多数miRNA在感染组中表达下调。miR-92a和miR-92b-3p在秋海棠毒株感染的样本中上调。对感染期间所有这些过表达的miRNA进行功能分析，发现它们与病毒感染过程和免疫反应相关的途径有关。此外，通过茎环RT-qPCR在体外和体内实验中均检测到8种病毒miRNA，呈现出一个影响59个病毒基因的基因调控网络。大多数已描述的病毒miRNA与大潜伏期转录本（LLT）、病毒转录激活因子EP0和IE180有关，也与调控基因有关，因为它们在病毒感染期间的宿主-病原体相互作用中发挥着重要作用。
