猪感染非洲猪瘟病毒后 microRNAs 的差异表达：概念验证研究。
Differential expression of porcine microRNAs in African swine fever virus infected pigs: a proof-of-concept study.
机构信息
IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Bellaterra, Spain.
Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba.
出版信息
Virol J. 2017 Oct 17;14(1):198. doi: 10.1186/s12985-017-0864-8.
BACKGROUND
African swine fever (ASF) is a re-expanding devastating viral disease currently threatening the pig industry worldwide. MicroRNAs are a class of 17-25 nucleotide non- coding RNAs that have been shown to have critical functions in a wide variety of biological processes, such as cell differentiation, cell cycle regulation, carcinogenesis, apoptosis, regulation of immunity as well as in viral infections by cleavage or translational repression of mRNAs. Nevertheless, there is no information about miRNA expression in an ASFV infection.
METHODS
In this proof-of-concept study, we have analyzed miRNAs expressed in spleen and submandibular lymph node of experimentally infected pigs with a virulent (E75) or its derived attenuated (E75CV1) ASFV strain, as well as, at different times post-infection with the virulent strain, by high throughput sequencing of small RNA libraries.
RESULTS
Spleen presented a more differential expression pattern than lymph nodes in an ASFV infection. Of the most abundant miRNAs, 12 were differentially expressed in both tissues at two different times in infected animals with the virulent strain. Of these, miR-451, miR-145-5p, miR-181a and miR-122 presented up-regulation at late times post-infection while miR-92a, miR-23a, miR-92b-3p, miR-126-5p, miR-126-3p, miR-30d, miR-23b and miR-92c showed down-regulation. Of the 8 differentially expressed miRNAs identified at the same time post-infection in infected animals with the virulent strain compared with animals infected with its attenuated strain, miR-126-5p, miR-92c, miR-92a, miR-30e-5p and miR-500a-5p presented up-regulation whereas miR-125b, miR-451 and miR-125a were down-regulated. All these miRNAs have been shown to be associated with cellular genes involved in pathways related to the immune response, virus-host interactions as well as with several viral genes.
CONCLUSION
The study of miRNA expression will contribute to a better understanding of African swine fever virus pathogenesis, essential in the development of any disease control strategy.
背景
非洲猪瘟（ASF）是一种重新扩张的毁灭性病毒病，目前正在威胁着全球的养猪业。miRNA 是一类 17-25 个核苷酸的非编码 RNA，已被证明在广泛的生物过程中具有关键功能，如细胞分化、细胞周期调控、癌变、细胞凋亡、免疫调节以及通过切割或翻译抑制 mRNA 来调节病毒感染。然而，目前尚无关于 ASF 感染中 miRNA 表达的信息。
方法
在这项概念验证研究中，我们通过高通量测序小 RNA 文库，分析了用强毒（E75）或其衍生的减毒（E75CV1）ASF 株感染的实验猪的脾脏和颌下淋巴结中表达的 miRNA。
结果
在 ASF 感染中，脾脏的差异表达模式比淋巴结更为明显。在感染动物的两种组织中，12 个最丰富的 miRNA 在感染强毒株的两个不同时间点均有差异表达。其中，miR-451、miR-145-5p、miR-181a 和 miR-122 在感染后晚期上调，而 miR-92a、miR-23a、miR-92b-3p、miR-126-5p、miR-126-3p、miR-30d、miR-23b 和 miR-92c 下调。在感染强毒株的动物与感染减毒株的动物在同一时间点鉴定出的 8 个差异表达 miRNA 中，miR-126-5p、miR-92c、miR-92a、miR-30e-5p 和 miR-500a-5p 上调，而 miR-125b、miR-451 和 miR-125a 下调。所有这些 miRNA 都与参与免疫反应、病毒-宿主相互作用以及与几种病毒基因相关的细胞基因有关。
结论
miRNA 表达的研究将有助于更好地了解非洲猪瘟病毒的发病机制，这对于制定任何疾病控制策略都是至关重要的。
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