Bollensen E, Schachner M
Department of Neurobiology, University of Heidelberg, F.R.G.
Neurosci Lett. 1987 Nov 10;82(1):77-82. doi: 10.1016/0304-3940(87)90174-1.
On the basis of recent evidence that the carbohydrate structures L2/HNK-1 and L3 are shared by several neural cell adhesion molecules, we have suggested that other glycoproteins carrying these carbohydrate structures are adhesion molecules. We have isolated P0, the major glycoprotein of peripheral myelin, from human sciatic nerve by immunoaffinity chromatography using a monoclonal L2 antibody column and tested it, along with bovine P0, in Western blots for the presence of the two carbohydrate structures. Here we show that P0 expresses the L2/HNK-1 and L3 carbohydrates.
基于最近的证据表明碳水化合物结构L2/HNK-1和L3为几种神经细胞粘附分子所共有，我们提出携带这些碳水化合物结构的其他糖蛋白也是粘附分子。我们通过使用单克隆L2抗体柱的免疫亲和层析从人坐骨神经中分离出外周髓磷脂的主要糖蛋白P0，并将其与牛P0一起在蛋白质印迹中检测这两种碳水化合物结构的存在。在此我们表明P0表达L2/HNK-1和L3碳水化合物。
