Weakley B S, Webb P, James J L
Cell Tissue Res. 1981;220(2):349-72. doi: 10.1007/BF00210514.
A cytochemical study of the Golgi apparatus in the developing oocyte of the golden hamster was carried out using the TPPase, AcPase and zinc iodide-osmium tetroxide (ZnOs) techniques. Tissue from both immature and sexually mature animals was investigated. Peak TPPase activity was found in pre-growth oocytes in ovaries from sexually mature adults. Some activity was also present in SER in the peripheral cytoplasm of growing oocytes. AcPase activity was found only after the onset of oocyte growth. It was present in Golgi cisternae and associated vesicles and in some profiles of peripheral SER. No structures corresponding to GERL were identified. Strong staining with ZnOs was seen, at all stages studied, in certain Golgi vesicles and short tubules but not in the cisternae unless the oocyte was atretic. Weaker ZnOs staining was characteristic of ER throughout the oocyte. With all techniques there was a falling off of reactivity as oocyte size increased. Within a single oocyte some Golgi bodies were negative while others were positive, with both TPPase and AcPase techniques. This suggests that two or more functional types of this organelle are present within the developing oocytes.
利用焦磷酸酶（TPPase）、酸性磷酸酶（AcPase）和碘化锌 - 四氧化锇（ZnOs）技术，对金黄仓鼠发育中卵母细胞的高尔基体进行了细胞化学研究。对未成熟和性成熟动物的组织都进行了研究。在性成熟成年动物卵巢中的生长前卵母细胞中发现了TPPase活性峰值。在生长中卵母细胞的外周细胞质的滑面内质网（SER）中也存在一些活性。AcPase活性仅在卵母细胞开始生长后才被发现。它存在于高尔基体潴泡和相关小泡以及外周SER的一些轮廓中。未鉴定出与GERL相对应的结构。在所有研究阶段，在某些高尔基体小泡和短小管中可见强烈的ZnOs染色，但在潴泡中未见，除非卵母细胞发生闭锁。较弱的ZnOs染色是整个卵母细胞内质网的特征。使用所有技术时，随着卵母细胞大小的增加，反应性会下降。在单个卵母细胞内，一些高尔基体呈阴性，而另一些呈阳性，这在TPPase和AcPase技术中均如此。这表明在发育中的卵母细胞内存在两种或更多种该细胞器的功能类型。
