BC Centre for Excellence in HIV/AIDS, Vancouver, British Columbia, Canada.
J Clin Microbiol. 2014 Feb;52(2):517-23. doi: 10.1128/JCM.02461-13. Epub 2013 Dec 4.
Low-level viremia during antiretroviral therapy and its accurate measurement are increasingly relevant. Here, we present an international collaboration of 4,221 paired blood plasma viral load (pVL) results from four commercial assays, emphasizing the data with low pVL. The assays compared were the Abbott RealTime assay, the Roche Amplicor assay, and the Roche TaqMan version 1 and version 2 assays. The correlation between the assays was 0.90 to 0.97. However, at a low pVL, the correlation fell to 0.45 to 0.85. The observed interassay concordance was higher when detectability was defined as 200 copies/ml than when it was defined as 50 copies/ml. A pVL of ∼100 to 125 copies/ml by the TaqMan version 1 and version 2 assays corresponded best to a 50-copies/ml threshold with the Amplicor assay. Correlation and concordance between the viral load assays were lower at a low pVL. Clear guidelines are needed on the clinical significance of low-level viremia.
在抗逆转录病毒治疗期间，低水平病毒血症及其准确测量变得越来越重要。在这里，我们呈现了一个由 4221 对来自四个商业检测的血浆病毒载量（pVL）结果组成的国际合作，强调了低 pVL 的数据。比较的检测方法是 Abbott RealTime 检测法、罗氏 Amplicor 检测法，以及罗氏 TaqMan 版本 1 和版本 2 检测法。这些检测方法之间的相关性为 0.90 到 0.97。然而，在低 pVL 时，相关性下降到 0.45 到 0.85。当以 200 拷贝/ml 而不是以 50 拷贝/ml 作为检测下限定义时，观察到的组间一致性更高。TaqMan 版本 1 和版本 2 检测法中约 100 到 125 拷贝/ml 的 pVL 与 Amplicor 检测法中 50 拷贝/ml 的阈值最匹配。在低 pVL 时，病毒载量检测之间的相关性和一致性较低。需要明确关于低水平病毒血症的临床意义的指南。
