Lee Hye Ryun, Shin Sue, Yoon Jong Hyun, Roh Eun Youn, Song Eun Young, Han Kyou Sup, Kim Byoung Jae
Department of Laboratory Medicine, Gyeongsang National University Hospital, Jinju, Korea.
Cell Transplant. 2015;24(2):305-10. doi: 10.3727/096368914X678544. Epub 2014 Jan 29.
A contiguous segment attached to the cord blood unit (CBU) is required for verifying HLA types, cell viability, and, possibly, potency before transplantation since such a segment is considered to be representative of the CBU. However, little is known regarding the characteristics of contiguous segments in comparison to main bag units due to the difficulty experienced in accessing a large number of cryopreserved CBUs. In this study, we used 245 nonconforming CBUs for allogeneic transplantation. After thawing the cryopreserved CBU, the number of total nucleated cells (TNCs), CD34(+) cells, and CFUs in CB from main bags and segments, as well as cell viability and apoptosis, were examined. The comparative analysis showed that the number of TNCs was significantly higher in CB from main bags, whereas the numbers of CD34(+) cells and CFU-GM were significantly higher in CB from segments. While the cell viability of TNCs in segments was higher, the proportion of apoptotic TNCs was also higher. In contrast, no difference was observed between the proportion of apoptotic CD34(+) cells in main bags and segments. In the correlation analysis, the numbers of TNCs, CD34(+) cells, and CFU-GM in main bags were highly correlated with those in segments, indicating that CB from segments is indeed representative of CB in main bags. Taken together, we conclude that segments have higher CD34(+) cells and CFU-GM and lower TNCs than the main cryopreserved bag, although the two compartments are highly correlated with each other.
由于在移植前需要一段与脐带血单位（CBU）相连的片段来验证人类白细胞抗原（HLA）类型、细胞活力以及可能的效力，因为该片段被认为是CBU的代表。然而，由于难以获取大量冷冻保存的CBU，与主袋单位相比，关于相连片段的特征知之甚少。在本研究中，我们使用了245个不符合标准的CBU进行异基因移植。解冻冷冻保存的CBU后，检测了主袋和片段中CB的总核细胞（TNCs）、CD34(+)细胞和集落形成单位（CFUs）的数量，以及细胞活力和凋亡情况。比较分析表明，主袋中CB的TNCs数量显著更高，而片段中CB的CD34(+)细胞和CFU-GM数量显著更高。虽然片段中TNCs的细胞活力更高，但凋亡TNCs的比例也更高。相比之下，主袋和片段中凋亡CD34(+)细胞的比例没有差异。在相关性分析中，主袋中TNCs、CD34(+)细胞和CFU-GM的数量与片段中的数量高度相关，表明片段中的CB确实是主袋中CB的代表。综上所述，我们得出结论，尽管两个部分彼此高度相关，但片段中的CD34(+)细胞和CFU-GM比冷冻保存的主袋更高，而TNCs更低。
