Seo Soo Hyun, Shin Sue, Roh Eun Youn, Song Eun Young, Oh Sohee, Kim Byoung Jae, Yoon Jong Hyun
Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea.
Ann Lab Med. 2017 Mar;37(2):124-128. doi: 10.3343/alm.2017.37.2.124.
Maintaining the quality of cryopreserved cord blood is crucial. In this pilot study, we describe the results of the internal quality control program for a cord blood bank thus far.
Donated cord blood units unsuitable for transplantation were selected for internal quality control once a month. One unit of cord blood, aliquoted into 21 capillaries, was cryopreserved and thawed annually to analyze the total nucleated cell count, CD34⁺ cell count, cell viability test, and colony-forming units assay.
No significant differences in the variables (total nucleated cell count, cell viability, CD34⁺ cell count) were observed between samples cryopreserved for one and two years. Upon comparing the variables before cryopreservation and post thawing with the capillaries of one year of storage, cell viability and CD34⁺ cell counts decreased significantly. The use of cord blood samples in capillaries, which can be easily stored for a long period, was similar to the methods used for testing segments attached to the cord blood unit.
The results of this study may be useful for determining the period during which the quality of cryopreserved cord blood units used for transplantation is maintained.
维持冷冻保存脐带血的质量至关重要。在这项初步研究中，我们描述了迄今为止一家脐带血库内部质量控制计划的结果。
每月选择一次不适合移植的捐赠脐带血单位用于内部质量控制。将一个脐带血单位分装到21根毛细管中，每年进行冷冻保存和解冻，以分析总核细胞计数、CD34⁺细胞计数、细胞活力测试和集落形成单位测定。
冷冻保存一年和两年的样本之间，各项变量（总核细胞计数、细胞活力、CD34⁺细胞计数）未观察到显著差异。将冷冻保存前和解冻后的变量与储存一年的毛细管样本进行比较时，细胞活力和CD34⁺细胞计数显著下降。使用易于长期储存的毛细管中的脐带血样本，与用于检测脐带血单位附着段的方法类似。
本研究结果可能有助于确定用于移植的冷冻保存脐带血单位质量得以维持的时间段。
