Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo, Hokkaido 001-0020, Japan.
Department of Microbiology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea.
Comp Immunol Microbiol Infect Dis. 2014 Mar;37(2):123-30. doi: 10.1016/j.cimid.2014.01.003. Epub 2014 Jan 15.
The Republic of Zambia consists of only one veterinary teaching school at the University of Zambia (UNZA) where students and veterinarians are exposed to many bacterial pathogens including Staphylococcus aureus (SA) and Staphylococcus pseudintermedius (SP). The aim of this study was the characterization and antimicrobial susceptibility profile of eleven SA and 48 SP isolates from the veterinary hospitals' in- and outpatients and the environment. No isolate was resistant to cefoxitin by disk diffusion test and the corresponding resistance gene mecA was not found. In contrast, the resistance rates of SA to penicillin (63.6%) and trimethoprim-sulfamethoxazole (36.4%) and SP to penicillin (52.1%) and tetracycline (25.0%) were the highest. A variety of sequence types (STs) without a predominant type including numerous novel types were determined, especially for SP (39.6%). The spa typing provided a clonal assignment for all SAs (100%) and 24 SPs (50%) with three and two novel types, respectively. This study has provided an overview of SA and SP in the veterinary teaching hospital at UNZA. However, for a better understanding of these species regarding pathogenesis and transmission, further studies on the prevalence and characterization of SA and SP from veterinary staff, pet owners, and farm animals in Zambia is needed.
赞比亚共和国只有一所位于赞比亚大学（UNZA）的兽医教学学校，学生和兽医在这里接触到许多细菌病原体，包括金黄色葡萄球菌（SA）和中间葡萄球菌（SP）。本研究的目的是对来自兽医医院门诊和住院患者及环境的 11 株 SA 和 48 株 SP 分离株进行特征描述和抗菌药物敏感性分析。没有分离株对头孢西丁纸片扩散法检测表现出耐药性，也未发现相应的耐药基因 mecA。相比之下，SA 对青霉素（63.6%）和复方磺胺甲噁唑（36.4%）以及 SP 对青霉素（52.1%）和四环素（25.0%）的耐药率最高。确定了多种无优势型别的序列型（ST），包括许多新型别，尤其是 SP（39.6%）。spa 分型为所有 SA（100%）和 24 株 SP（50%）提供了克隆分配，分别有 3 种和 2 种新型别。本研究概述了 UNZA 兽医教学医院的 SA 和 SP 情况。然而，为了更好地了解这些物种的发病机制和传播方式，有必要对赞比亚兽医工作人员、宠物主人和农场动物的 SA 和 SP 的流行情况和特征进行进一步研究。
