Exadaktylos P, Schlette O, Rumler W, Weidhase R, Haerting J, Reiss T, Heinrichs I
Klinik und Poliklinik für Kinderheilkunde des Bereiches Medizin, Martin-Luther-Universität Halle, Wittenberg.
Folia Haematol Int Mag Klin Morphol Blutforsch. 1987;114(5):617-23.
The DNA-content of mononuclear cells of the peripheral blood of infantile and juvenile ALL patients was investigated using Pulse Cytophotometry. The fraction of cells in S- and G2 + M-phase is significantly increased in comparison with samples of healthy probands. The fraction of DNA-synthesising cells (S-phase) of both peripheral blood (mononuclear cells) and bone marrow of leukemia patients cannot be significantly distinguished by mathematical methods. On the other hand, the fraction of cells in later phases of cell cycle (G2 + M-phase) is significant enhanced in the bone marrow in comparison with the peripheral blood. A high correlation was found between the number of leukocytes and fraction of G2 + M-phase cells in the peripheral blood of SR- and MR-patients. No correlation was found between the number of leukocytes and S-phase-fraction. The occurrence of aneuploid cell populations in the mononuclear fraction of peripheral blood in the acute state of ALL could be of importance for prognosis and regime of therapy.
采用脉冲细胞光度法研究了婴幼儿和青少年急性淋巴细胞白血病（ALL）患者外周血单个核细胞的DNA含量。与健康对照样本相比，S期和G2+M期细胞比例显著增加。白血病患者外周血（单个核细胞）和骨髓中DNA合成细胞（S期）的比例无法通过数学方法显著区分。另一方面，与外周血相比，骨髓中细胞周期后期（G2+M期）的细胞比例显著增加。在SR和MR患者的外周血中，白细胞数量与G2+M期细胞比例之间存在高度相关性。白细胞数量与S期比例之间未发现相关性。ALL急性期外周血单个核细胞部分非整倍体细胞群的出现可能对预后和治疗方案具有重要意义。
