通过纳米线修饰的微电极安培法实时监测植物原生质体中生长素小泡胞吐的排出。

Real-time monitoring of auxin vesicular exocytotic efflux from single plant protoplasts by amperometry at microelectrodes decorated with nanowires.

机构信息

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).

出版信息

Angew Chem Int Ed Engl. 2014 Mar 3;53(10):2643-7. doi: 10.1002/anie.201308972. Epub 2014 Jan 30.

DOI:10.1002/anie.201308972

PMID:24482020

Abstract

Recent biochemical results suggest that auxin (IAA) efflux is mediated by a vesicular cycling mechanism, but no direct detection of vesicular IAA release from single plant cells in real-time has been possible up to now. A TiC@C/Pt-QANFA micro-electrochemical sensor has been developed with high sensitivity in detection of IAA, and it allows real-time monitoring and quantification of the quantal release of auxin from single plant protoplast by exocytosis.

摘要

最近的生化研究结果表明，生长素（IAA）外排是通过囊泡循环机制介导的，但迄今为止，还不可能直接实时检测到单个植物细胞中囊泡释放的 IAA。本文制备了一种 TiC@C/Pt-QANFA 微电化学传感器，对 IAA 的检测具有高灵敏度，可通过胞吐作用实时监测和定量单个植物原生质体中生长素的量子释放。