O'Neil C E, Hughes J M, Butcher B T, Salvaggio J E, Lehrer S B
Department of Medicine, Tulane Medical Center, New Orleans, La.
Int Arch Allergy Appl Immunol. 1988;85(2):161-6. doi: 10.1159/000234496.
Spore extracts, prepared from Armillariella tabescens, Pleurotus ostreatus, Coprinus quadrifidus, Amanita muscaria, Ganoderma lucidum, Psilocybe cubensis, Pisolithus tinctorius, Scleroderma sp. and Calvatia cyathiformis, were examined for antigenic/allergenic relationships by Ouchterlony and radioallergosorbent testing (RAST) inhibition, respectively. Ouchterlony, using hyperimmunized rabbit sera, demonstrated a high degree of cross-antigenicity among the extracts tested; however, some unique antigens were also present. RAST inhibition, evaluated by comparing extract concentrations which inhibited the RAST by 50% (IC-50), varied with the allergen tested. P. cubensis was the most potent inhibitor (IC-50 ranged from 0.034 mg/ml for A. tabescens RAST to 0.29 mg/ml for G. lucidum RAST). P. tinctorius was the least potent inhibitor, failing to reach IC-50 at 10 mg/ml for any basidiospore extract. Evaluation of slopes and intercepts of the dose-response lines demonstrated qualitative and quantitative differences among allergens in these extracts. These results indicate the presence of shared allergenic epitopes, and suggest that representative extract panels could be developed for future use in diagnosis and treatment of basidiospore-sensitive individuals.
分别采用免疫双扩散法和放射变应原吸附试验（RAST）抑制试验，检测了从亮菌、糙皮侧耳、毛头鬼伞、毒蝇鹅膏、灵芝、古巴裸盖菇、彩色豆马勃、硬皮马勃属和梨形马勃制备的孢子提取物的抗原/变应原关系。免疫双扩散法使用经高度免疫的兔血清，结果显示受试提取物之间存在高度交叉抗原性；然而，也存在一些独特的抗原。通过比较使RAST抑制50%（IC-50）的提取物浓度来评估RAST抑制试验，其结果因受试变应原而异。古巴裸盖菇是最有效的抑制剂（IC-50范围从对亮菌RAST的0.034毫克/毫升到对灵芝RAST的0.29毫克/毫升）。彩色豆马勃是最无效的抑制剂，对于任何担子孢子提取物，在10毫克/毫升时都未达到IC-50。对剂量反应线的斜率和截距的评估表明这些提取物中的变应原在定性和定量上存在差异。这些结果表明存在共同的变应原表位，并提示可为未来用于诊断和治疗对担子孢子敏感的个体开发代表性提取物组。
