Liengswangwong V, Salvaggio J E, Lyon F L, Lehrer S B
Clin Allergy. 1987 May;17(3):191-8. doi: 10.1111/j.1365-2222.1987.tb02003.x.
Five methods were tested by RAST for allergen extraction from seven basidiospore species Armillaria tabescens, Chlorophyllum molybdites, Coprinus quadrifidus, Pleurotus ostreatus, Calvatia cyathiformis, Pisolithus tinctorius, and Scleroderma sp. With each basidiospore type, extracted allergen activity varied according to the method employed. In general, defatting of spores with ethyl ether, followed by homogenization in 0.125 M NH4HCO3 buffer, resulted in greatest allergen yield. In order to compare extracts from different isolates, batches of Pleurotus ostreatus spores obtained from different locations and over different time periods were analysed. Spores harvested from the same basidiomycete species in different areas varied in allergen (RAST) and protein (HPLC profile) content. The spores obtained from the same location over a 1-2 year period did not differ significantly. These results indicate that basidiospores can be stored for several years and that spore extracts from different locations can vary. These studies will help in the future to provide better characterized extracts for clinical studies.
通过放射性变应原吸附试验（RAST）测试了五种从七种担子孢子菌中提取变应原的方法，这七种担子孢子菌分别是：亮菌、铅绿褶菇、四孢鬼伞、平菇、马勃、彩色豆马勃和硬皮马勃属。对于每种担子孢子类型，提取的变应原活性会因所采用的方法而有所不同。一般来说，先用乙醚对孢子进行脱脂处理，然后在0.125M碳酸氢铵缓冲液中匀浆，可获得最高的变应原产量。为了比较不同分离株的提取物，对从不同地点和不同时间段获得的平菇孢子批次进行了分析。从不同地区同一担子菌种收获的孢子，其变应原（RAST）和蛋白质（高效液相色谱图谱）含量存在差异。在1 - 2年期间从同一地点获得的孢子没有显著差异。这些结果表明，担子孢子可以保存数年，并且不同地点的孢子提取物可能会有所不同。这些研究将有助于未来为临床研究提供特征更明确的提取物。
