Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA.
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Lab Chip. 2017 Mar 14;17(6):1137-1148. doi: 10.1039/c6lc01450j.
The analysis of tear constituents at point-of-care settings has a potential for early diagnosis of ocular disorders such as dry eye disease, low-cost screening, and surveillance of at-risk subjects. However, current minimally-invasive rapid tear analysis systems for point-of-care settings have been limited to assessment of osmolarity or inflammatory markers and cannot differentiate between dry eye subclassifications. Here, we demonstrate a portable microfluidic system that allows quantitative analysis of electrolytes in the tear fluid that is suited for point-of-care settings. The microfluidic system consists of a capillary tube for sample collection, a reservoir for sample dilution, and a paper-based microfluidic device for electrolyte analysis. The sensing regions are functionalized with fluorescent crown ethers, o-acetanisidide, and seminaphtorhodafluor that are sensitive to mono- and divalent electrolytes, and their fluorescence outputs are measured with a smartphone readout device. The measured sensitivity values of Na, K, Ca ions and pH in artificial tear fluid were matched with the known ion concentrations within the physiological range. The microfluidic system was tested with samples having different ionic concentrations, demonstrating the feasibility for the detection of early-stage dry eye, differential diagnosis of dry eye sub-types, and their severity staging.
在即时检测点，对泪液成分进行分析具有早期诊断眼部疾病（如干眼症）、低成本筛查和监测高危人群的潜力。然而，目前用于即时检测点的微创型快速泪液分析系统仅局限于评估渗透压或炎症标志物，无法区分干眼症的亚类。在这里，我们展示了一种适用于即时检测点的便携式微流控系统，该系统可对泪液中的电解质进行定量分析。微流控系统由用于样品采集的毛细管、用于样品稀释的储液器以及用于电解质分析的基于纸张的微流控器件组成。感应区域用荧光冠醚、邻乙酰替甲氧基苯胺和 seminaphtorhodafluor 进行功能化，这些物质对单价和二价电解质敏感，其荧光输出由智能手机读取设备进行测量。在人工泪液中，Na+、K+、Ca2+离子和 pH 值的测量灵敏度值与生理范围内已知的离子浓度相匹配。该微流控系统已用具有不同离子浓度的样品进行了测试，证明了其在早期干眼症检测、干眼症亚型的鉴别诊断及其严重程度分期方面的可行性。
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