Becker A B, Chung F, McDonald D M, Frick O L, Gold W M
Cardiovascular Research Institute, University of California, San Francisco 94143-0130.
J Allergy Clin Immunol. 1988 Feb;81(2):441-8. doi: 10.1016/0091-6749(88)90914-1.
We studied the cutaneous response to intradermal antigen using clinical, histologic, and physiologic criteria in ragweed-sensitized dogs. The clinical response was measured early (the wheal at 20 minutes) and late (induration at 6 hours). We assessed cutaneous responsiveness to histamine before and 6 hours after injection (intradermally) of ragweed (n = 5, antigen group) and diluent (n = 4, 10% glycerin in 0.9% NaCl, sham group); we measured the wheal in response to histamine (1.0 ng to 0.1 mg, intradermally), constructed a dose-response curve, and interpolated the provocative dose (milligrams) of histamine required to create a wheal 10 mm larger than the response to saline control. Skin biopsy specimens were obtained before and after injection of either ragweed or diluent. Consistent with the human late-phase response, neutrophils and eosinophils were present in the dermis at 1 hour, maximal at 6 hours, and decreased at 24 hours. Mononuclear cells increased significantly at 6 hours and were the predominant cells present at 24 hours after antigen. The late clinical response correlated only with influx of eosinophils (rs = 0.85; p less than 0.005). Histamine responsiveness increased markedly after antigen (p less than 0.0001), did not change after glycerin diluent (sham), and was correlated with the intensity of neutrophil influx at 6 hours (rs = 0.69; p less than 0.05), and to a much greater degree with mononuclear cell influx at 6 hours (rs = 0.85; p less than 0.005).
我们使用临床、组织学和生理学标准，研究了豚草致敏犬对皮内抗原的皮肤反应。早期（20分钟时的风团）和晚期（6小时时的硬结）测量临床反应。我们评估了在注射（皮内）豚草（n = 5，抗原组）和稀释剂（n = 4，0.9%氯化钠中的10%甘油，假手术组）之前和之后6小时皮肤对组胺的反应性；我们测量了对组胺（1.0 ng至0.1 mg，皮内）的风团反应，构建了剂量反应曲线，并内插产生比生理盐水对照反应大10 mm的风团所需的组胺激发剂量（毫克）。在注射豚草或稀释剂之前和之后获取皮肤活检标本。与人类迟发反应一致，中性粒细胞和嗜酸性粒细胞在1小时时出现在真皮中，6小时时达到峰值，24小时时减少。单核细胞在6小时时显著增加，是抗原注射后24小时时的主要细胞。晚期临床反应仅与嗜酸性粒细胞的流入相关（rs = 0.85；p < 0.005）。抗原注射后组胺反应性显著增加（p < 0.0001），甘油稀释剂（假手术）注射后无变化，且与6小时时中性粒细胞流入强度相关（rs = 0.69；p < 0.05），在更大程度上与6小时时单核细胞流入相关（rs = 0.85；p < 0.005）。
