Escuela de Quimica and CIPRONA, Universidad de Costa Rica , 2060 San José, Costa Rica.
J Nat Prod. 2014 Mar 28;77(3):455-63. doi: 10.1021/np400672g. Epub 2014 Jan 31.
The leaves of Zuelania guidonia yielded eight new clerodane diterpenes, namely, zuelaguidins A-H (1-8), and the known clerodane diterpene esculentin A (9). Some of these structures contained a 3,6-dihydro-1,2-dioxin moiety. The new compounds were isolated and identified using 1D- and 2D-NMR experiments. All compounds were evaluated for cytotoxicity against the CCRF-CEM (human acute lymphocytic leukemia), CEM-ADR5000 (human acute lymphocytic leukemia resistant to doxorubicin), and MIA-PaCa-2 (human pancreatic carcinoma) cell lines as well as for their selectivity against peripheral blood mononuclear cells from healthy human subjects. Zuelaguidins B, C, and E were the most potent compounds against the CCRF-CEM cell line, with IC50 values ranging from 1.6 to 2.5 μM.
紫堇属 Guidonia 的叶子产生了 8 种新的克氏二萜，分别为 zuelaguidins A-H（1-8）和已知的克氏二萜 esculentin A（9）。其中一些结构含有 3,6-二氢-1,2-二恶烷部分。新化合物是通过 1D 和 2D-NMR 实验分离和鉴定的。所有化合物均针对 CCRF-CEM（人急性淋巴细胞白血病）、CEM-ADR5000（对阿霉素耐药的人急性淋巴细胞白血病）和 MIA-PaCa-2（人胰腺癌细胞系）进行了细胞毒性评估，并针对健康人外周血单个核细胞进行了选择性评估。Zuelaguidins B、C 和 E 对 CCRF-CEM 细胞系的活性最强，IC50 值范围为 1.6 至 2.5 μM。
