克罗藤 oligandrus 皮埃尔 ex 哈钦。树皮中的 Ent-Clerodane 二萜类化合物及其对人癌细胞系的细胞毒性评价。
Ent-Clerodane Diterpenes from the Bark of Croton oligandrus Pierre ex Hutch. and Assessment of Their Cytotoxicity against Human Cancer Cell Lines.
机构信息
Medicinal Chemistry and Natural Products Research Group, School of Pharmacy and Biomolecular Sciences, Faculty of Science, Liverpool John Moores University, Liverpool L3 3AF, UK.
出版信息
Molecules. 2018 Feb 13;23(2):410. doi: 10.3390/molecules23020410.
New clerodane diterpenes, 12--megalocarpodolide D () and an epimeric mixture of crotonolins A () and B (), were isolated from the bark of following a bioassay-guided isolation protocol. Known compounds, megalocarpodolide D (), 12--crotocorylifuran (), cluytyl-ferulate (), hexacosanoyl- ferulate (), vanillin (), acetyl-aleuritolic acid () and lupeol (), were also isolated. The structures of the isolated compounds (-) were elucidated by spectroscopic means. The cytotoxicity of compounds - was assessed against A549, MCF7, PC3 and PNT2 cell lines using the MTT assay. Compounds and showed moderate levels of activity against both A549 and MCF7 cells with being the most active with IC values of 63.8 ± 13.8 and 136.2 ± 22.7 µM against A549 and MCF7 cells, respectively. The epimeric mixture of and was moderately active against A549 and PC3 cells (IC = 128.6 ± 31.0 and 111.2 ± 2.9 µM, respectively).
从Megocarpus fagelifolius 的树皮中，通过生物测定指导的分离方案，分离出了新的 clerodane 二萜，12--megalocarpodolide D () 和一个 crotonolins A () 和 B () 的非对映异构体混合物。还分离出了已知化合物 megalocarpodolide D (), 12--crotocorylifuran (), cluytyl-ferulate (), hexacosanoyl- ferulate (), vanillin (), acetyl-aleuritolic acid () 和 lupeol ()。通过光谱手段阐明了分离化合物 (-) 的结构。用 MTT 测定法评估了化合物 - 对 A549、MCF7、PC3 和 PNT2 细胞系的细胞毒性。化合物 和 对 A549 和 MCF7 细胞均表现出中等水平的活性，其中 最为活跃，对 A549 和 MCF7 细胞的 IC 值分别为 63.8 ± 13.8 和 136.2 ± 22.7 μM。和 的非对映异构体混合物对 A549 和 PC3 细胞具有中等活性（IC = 128.6 ± 31.0 和 111.2 ± 2.9 μM，分别）。
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