Portran Didier
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Methods Cell Biol. 2014;120:39-51. doi: 10.1016/B978-0-12-417136-7.00003-3.
The following protocol describes a method to control the orientation and polarity of polymerizing microtubules (MTs). Reconstitution of specific geometries of dynamic MT networks is achieved using a ultraviolet (UV) micropatterning technique in combination with stabilized MT microseeds. The process is described in three main parts. First, the surface is passivated to avoid the non-specific absorption of proteins, using different polyethylene glycol (PEG)-based surface treatment. Second, specific adhesive surfaces (the micropatterns) are imprinted through a photomask using deep UVs. Lastly, MT microseeds are adhered to the micropatterns followed by MT polymerization.
以下方案描述了一种控制聚合微管（MTs）方向和极性的方法。使用紫外线（UV）微图案化技术结合稳定的MT微种子，实现动态MT网络特定几何形状的重构。该过程主要分为三个部分。首先，使用不同的基于聚乙二醇（PEG）的表面处理方法对表面进行钝化，以避免蛋白质的非特异性吸附。其次，通过光掩膜使用深紫外线印出特定的粘附表面（微图案）。最后，将MT微种子粘附到微图案上，随后进行MT聚合。
