Eikmanns Bernhard J, Blombach Bastian
Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany.
Institute of Biochemical Engineering, University of Stuttgart, 70569 Stuttgart, Germany.
J Biotechnol. 2014 Dec 20;192 Pt B:339-45. doi: 10.1016/j.jbiotec.2013.12.019. Epub 2014 Jan 29.
The pyruvate dehydrogenase complex (PDHC) catalyzes the oxidative thiamine pyrophosphate-dependent decarboxylation of pyruvate to acetyl-CoA and CO2. Since pyruvate is a key metabolite of the central metabolism and also the precursor for several relevant biotechnological products, metabolic engineering of this multienzyme complex is a promising strategy to improve microbial production processes. This review summarizes the current knowledge and achievements on metabolic engineering approaches to tailor the PDHC of Corynebacterium glutamicum for the bio-based production of l-valine, 2-ketosiovalerate, pyruvate, succinate and isobutanol and to improve l-lysine production.
丙酮酸脱氢酶复合体（PDHC）催化以硫胺素焦磷酸为辅酶的丙酮酸氧化脱羧反应，生成乙酰辅酶A和二氧化碳。由于丙酮酸是中心代谢的关键代谢物，也是几种相关生物技术产品的前体，对这种多酶复合体进行代谢工程改造是改善微生物生产过程的一种很有前景的策略。本文综述了通过代谢工程方法对谷氨酸棒杆菌的PDHC进行改造，用于生物法生产L-缬氨酸、2-酮异戊酸、丙酮酸、琥珀酸和异丁醇以及提高L-赖氨酸产量的相关知识和研究成果。
