评估自动化 SPF10-LiPA25 检测和分型方法在存档样本中检测人乳头瘤病毒的效果。
Evaluation of an automated SPF10-LiPA25 assay for detection and typing of human papillomavirus in archival samples.
机构信息
Regional HPV Labnet Reference Laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia; Murdoch Children's Research Institute, Parkville, Victoria, Australia.
University of Applied Sciences, Leiden, The Netherlands.
出版信息
J Virol Methods. 2014 Apr;199:116-8. doi: 10.1016/j.jviromet.2014.01.012. Epub 2014 Jan 30.
An automated blotter was evaluated for detection of the HPV SPF10-LiPA25 RHA for HPV genotyping with 63 FFPE tissue and 45 WHO proficiency panel samples. The results showed that 10-20% more types were detected and bands were of significantly stronger intensity (p<0.0001) compared to manual processing. Therefore it was concluded that automated detection of HPV genotypes using the HPV SPF10-LiPA25 is more sensitive for low copy number and mixed-type HPV samples.
评估了一种自动化印迹仪，用于检测 HPV SPF10-LiPA25 RHA 进行 HPV 基因分型，共检测了 63 个 FFPE 组织和 45 个 WHO 能力验证样本。结果表明，与手动处理相比，检测到的类型增加了 10-20%，并且条带的强度明显增强（p<0.0001）。因此，结论是使用 HPV SPF10-LiPA25 进行 HPV 基因型的自动化检测对于低拷贝数和混合型 HPV 样本更敏感。
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