Ziaee Shayan, Kalayinia Samira, Boroumand Mohammad A, Pourgholi Leyla, Cheraghi Sara, Anvari Maryam S, Sheikhvatan Mehrdad
Department of Pathology, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
Coron Artery Dis. 2014 May;25(3):242-6. doi: 10.1097/MCA.0000000000000075.
The atrial natriuretic peptide (ANP) gene expression and some of its related single-nucleotide polymorphisms have been well established as a characterized biomarker of cardiovascular diseases. In the present study, we aimed to evaluate the potential association between one of the introduced ANP gene polymorphisms of 2238 T/C (rs5065) with coronary artery disease (CAD) in an Iranian population.
A total of 573 patients with CAD according to angiography reports and 293 controls without any evidence of CAD were enrolled. Allelic discrimination of the single-nucleotide polymorphism rs5065 in both groups was performed using a High Resolution Melt technique in real-time PCR analysis.
With respect to the prevalence of different genotypes of rs5065 polymorphism, the frequency of the T allele in the CAD group was significantly lower in CAD than that in the non-CAD group (59.7 vs. 65.1%, P=0.032). A significant inverse association was also found between the frequency of T allele and severity of CAD assessed by the Gensini score; the average of this score in T-allele carriers was 38.6±41.6 and that in C-allele carriers was 57.7±46.3 (P≤0.0001). Using multivariable linear regression modeling with the presence of baseline variables, the presence of the rs5065 ANP T allele could predict decreased severity of CAD assessed by the Gensini score in our population.
The presence of the rs5065 ANP polymorphism is potentially associated with a reduced risk of CAD as well as with reduced severity of CAD independent of the general risk factors of CAD.
心房利钠肽（ANP）基因表达及其一些相关单核苷酸多态性已被充分确立为心血管疾病的特征性生物标志物。在本研究中，我们旨在评估伊朗人群中ANP基因2238 T/C（rs5065）的一种引入的单核苷酸多态性与冠状动脉疾病（CAD）之间的潜在关联。
根据血管造影报告，共纳入573例CAD患者和293例无CAD证据的对照。在实时PCR分析中，使用高分辨率熔解技术对两组中的单核苷酸多态性rs5065进行等位基因鉴别。
关于rs5065多态性不同基因型的患病率，CAD组中T等位基因的频率显著低于非CAD组（59.7%对65.1%，P = 0.032）。在通过Gensini评分评估的CAD严重程度与T等位基因频率之间也发现了显著的负相关；T等位基因携带者的该评分平均值为38.6±41.6，C等位基因携带者为57.7±46.3（P≤0.0001）。使用包含基线变量的多变量线性回归模型，rs5065 ANP T等位基因的存在可预测我们人群中通过Gensini评分评估的CAD严重程度降低。
rs5065 ANP多态性的存在可能与CAD风险降低以及CAD严重程度降低相关，且独立于CAD的一般风险因素。
