College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Biol Trace Elem Res. 2014 Mar;157(3):234-41. doi: 10.1007/s12011-014-9893-y. Epub 2014 Feb 2.
Previous studies have determined the effects of dietary selenium (Se) supplementation on selenoprotein N (SelN, SEPN1), selenophosphate synthetase-1 (SPS1), and selenocysteine-synthase (SecS) mRNA abundance in chicken skeletal and cardiac muscles. To investigate collective responses of these genes to dietary Se concentrations ranging from deficiency to moderately high level in muscle tissues of chicken, 1-day-old chickens were exposed to a diet of deficient Se and supplemented with Se (0.15 mg Se/kg and 1.50 mg Se/kg) as sodium selenite in the feed for 35 days. Muscle tissues (flight, breast, leg, and cardiac muscles) were collected and examined for Se content and mRNA levels of SelN on days 1, 15, 25, and 35 days, respectively. Moreover, SPS1 and SecS mRNA levels were analyzed. The results showed that the expression of SelN gene in cardiac muscle responded to dietary Se concentrations. SelN gene was downregulated in the Se deficiency group (L group), and upregulated in the Se excess group (H group) compared with the moderate Se group (M group) (P < 0.05) in cardiac muscle. Se deficiency mainly unregulated SelN mRNA level in skeletal muscles compared with M group. Excess dietary Se mainly resulted in the upregulation of SelN mRNA level in skeletal muscles compared with the M group. SecS mRNA levels responded to dietary Se concentrations showed a similar change compared with SelN in cardiac muscle. SPS1 mRNA levels responded to dietary Se concentrations showed a downregulation in L group and upregulation in H group. However, SelN mRNA levels displayed a different expression pattern in different skeletal and cardiac muscles. Moreover, Se also regulated the levels of SPS1 and SecS mRNAs. In summary, Se regulated the expression of SelN gene and affected the mRNA levels of SecS and SPS1. The level of Se in the feed may regulate SelN biosynthesis by affecting the levels of SPS1 and SecS mRNA.
先前的研究已经确定了膳食硒（Se）补充对鸡骨骼肌和心肌中硒蛋白 N（SelN，SEPN1）、磷酸吡哆醛合成酶-1（SPS1）和硒代半胱氨酸合成酶（SecS）mRNA 丰度的影响。为了研究这些基因对鸡肌肉组织中从缺乏到中等高水平的膳食 Se 浓度的集体反应，将 1 日龄鸡暴露于缺乏 Se 的饮食中，并在饲料中添加 Se（0.15 mg Se/kg 和 1.50 mg Se/kg）作为亚硒酸钠，共 35 天。分别在第 1、15、25 和 35 天收集肌肉组织（飞行肌、胸肌、腿肌和心肌）并检测 Se 含量和 SelN 的 mRNA 水平。此外，还分析了 SPS1 和 SecS mRNA 水平。结果表明，心肌中 SelN 基因的表达对膳食 Se 浓度有反应。与中等 Se 组（M 组）相比，在 Se 缺乏组（L 组）中 SelN 基因下调，在 Se 过量组（H 组）中上调（P<0.05）。与 M 组相比，Se 缺乏主要调节骨骼肌中的 SelN mRNA 水平。与 M 组相比，过量的膳食 Se 主要导致骨骼肌中 SelN mRNA 水平上调。与 SelN 相似，心肌中 SecS mRNA 水平对膳食 Se 浓度的反应表现出相似的变化。SPS1 mRNA 水平对膳食 Se 浓度的反应在 L 组中下调，在 H 组中上调。然而，SelN mRNA 水平在不同的骨骼肌和心肌中表现出不同的表达模式。此外，Se 还调节了 SPS1 和 SecS mRNAs 的水平。总之，Se 调节 SelN 基因的表达，影响 SecS 和 SPS1 的 mRNA 水平。饲料中的 Se 水平可能通过影响 SPS1 和 SecS mRNA 的水平来调节 SelN 生物合成。
