Lomonte B, Moreno E, Gutiérrez J M
Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José.
Toxicon. 1987;25(9):947-55. doi: 10.1016/0041-0101(87)90157-7.
The presence of components antigenically related to Bothrops asper myotoxin was investigated by Western blotting and immunoelectrophoretic techniques. B. asper myotoxin is a non-glycosylated monomeric phospholipase A with a molecular weight by SDS-PAGE of 16,000 and isoelectric point of pH 9.8-10.0. Results showed that proteins in the venoms of B. nummifer, B. godmani, B. schlegelii, B. picadoi, and Agkistrodon bilineatus were recognized by monospecific antibodies to B. asper myotoxin raised in rabbit and sheep. Western blotting indicated that cross-reacting proteins have a molecular weight of 16,000, with the exception of that of B. picadoi, which is of 24,000 mol. wt. However, immunoelectrophoresis indicated that these components are highly heterogeneous in charge, ranging from basic to acidic proteins. The cross-reacting component(s) present in newborn B. asper venom has a different charge from that of the 'adult-type'. Venoms from newborn specimens showed an additional cross-reacting band of 18,000 mol. wt. Myotoxin is an abundant component in adult B. asper venom. Myotoxin-antimyotoxin complexes had different electrophoretic mobilities in rocket immunoelectrophoresis depending upon the species in which monospecific immune sera were produced.
通过蛋白质印迹法和免疫电泳技术研究了与矛头蝮肌毒素抗原相关的成分的存在情况。矛头蝮肌毒素是一种非糖基化的单体磷脂酶A，通过SDS-PAGE测定其分子量为16,000，等电点为pH 9.8 - 10.0。结果表明，兔和羊体内产生的针对矛头蝮肌毒素的单特异性抗体识别出了矛头蝮、墨西哥矛头蝮、施氏矛头蝮、皮氏矛头蝮和双带咝蝰毒液中的蛋白质。蛋白质印迹法表明，除皮氏矛头蝮的交叉反应蛋白分子量为24,000外，其他交叉反应蛋白的分子量均为16,000。然而，免疫电泳表明这些成分在电荷方面高度异质，从碱性蛋白到酸性蛋白都有。新生矛头蝮毒液中存在的交叉反应成分与“成年型”的电荷不同。新生标本的毒液显示出一条额外的分子量为18,000的交叉反应带。肌毒素是成年矛头蝮毒液中的一种丰富成分。肌毒素-抗肌毒素复合物在火箭免疫电泳中的电泳迁移率因产生单特异性免疫血清的物种而异。
