Vrati S, Fernon C A, Dalgarno L, Weir R C
Department of Biochemistry, Faculty of Science, Australian National University, Canberra.
Virology. 1988 Feb;162(2):346-53. doi: 10.1016/0042-6822(88)90474-6.
The location of a major antigenic domain involved in the neutralization of an alphavirus, Ross River virus, has been defined in terms of its position in the amino acid sequence of the E2 glycoprotein. The domain encompasses three topographically close epitopes which were identified using three E2-specific neutralizing monoclonal antibodies in competitive binding assays. Nucleotide sequencing of the structural protein genes of monoclonal antibody-selected antigenic variants showed that for each variant there was a single nucleotide change in the E2 gene leading to a nonconservative amino acid substitution in E2. Changes were at positions 216, 234, and 246-251 in the amino acid sequence. The epitopes are in a region of E2 which, though not strongly conserved as to sequence among Ross River virus, Semliki Forest virus, and Sindbis virus, is conserved in its hydropathy profile among the three alphaviruses. The epitopes lie between two asparagine-linked glycosylation sites (residues 200 and 262) in E2. They are conserved as to position between the mouse virulent T48 strain and the mouse avirulent NB5092 strain.
在甲病毒罗斯河病毒（Ross River virus）中和过程中起作用的一个主要抗原结构域，已根据其在E2糖蛋白氨基酸序列中的位置得以确定。该结构域包含三个在拓扑结构上相邻的表位，这些表位是在竞争结合试验中使用三种E2特异性中和单克隆抗体鉴定出来的。对单克隆抗体选择的抗原变异体的结构蛋白基因进行核苷酸测序表明，对于每个变异体，E2基因中都有一个单核苷酸变化，导致E2中出现一个非保守氨基酸取代。变化发生在氨基酸序列的第216、234和246 - 251位。这些表位位于E2的一个区域，该区域虽然在罗斯河病毒、辛德毕斯病毒（Semliki Forest virus）和辛德毕斯病毒（Sindbis virus）之间的序列保守性不强，但在这三种甲病毒之间其亲水性图谱是保守的。这些表位位于E2中两个天冬酰胺连接的糖基化位点（第200和262位残基）之间。它们在小鼠强毒株T48和小鼠无毒株NB5092之间的位置是保守的。
