Dalgarno L, Rice C M, Strauss J H
Virology. 1983 Aug;129(1):170-87. doi: 10.1016/0042-6822(83)90404-x.
The complete sequence of the 26 S RNA of Ross River virus (T48 strain) has been obtained and from this the amino acid sequences of the encoded structural proteins have been deduced. These include a basic capsid protein and two envelope glycoproteins. The nucleotide sequence was obtained by chemical sequence analysis of both single-stranded and double-stranded cDNA made to RNA and the sequence data so obtained was rapidly aligned by making use of the protein homology found among the alphaviruses. The polyprotein precursor encoded by the 26 S RNA of Ross River virus is 75% homologous to that of Semliki Forest virus and 48% homologous to that of Sindbis virus. The extent of homology is not uniform within a protein or between proteins and this is discussed with respect to the possible function of the various polypeptide domains in the virus life cycle. In each case the putative attachment site of the amino proximal carbohydrate chains of the three glycoproteins is conserved, whereas the attachment site of a second chain, if present, is not conserved. The 3'-untranslated region of Ross River virus RNA is 524 nucleotides long. It contains a sequence of about 50 nucleotides in length which is present in four copies but which is not shared with other alphaviruses examined.
已获得罗斯河病毒（T48株）26S RNA的完整序列，并由此推导了编码结构蛋白的氨基酸序列。这些结构蛋白包括一种碱性衣壳蛋白和两种包膜糖蛋白。通过对RNA的单链和双链cDNA进行化学序列分析获得了核苷酸序列，并利用甲病毒之间的蛋白质同源性对所得序列数据进行了快速比对。罗斯河病毒26S RNA编码的多蛋白前体与Semliki森林病毒的多蛋白前体有75%的同源性，与辛德比斯病毒的多蛋白前体有48%的同源性。同源程度在一种蛋白质内部或不同蛋白质之间并不一致，本文就此讨论了病毒生命周期中各种多肽结构域可能的功能。在每种情况下，三种糖蛋白氨基近端碳水化合物链的假定附着位点是保守的，而第二条链（如果存在）的附着位点则不保守。罗斯河病毒RNA的3'非翻译区有524个核苷酸长。它包含一段约50个核苷酸长的序列，该序列以四个拷贝形式存在，但在所检测的其他甲病毒中不存在。
