Li Xuankun, Li Wenliang, Ding Shuangmei, Cameron Stephen L, Mao Meng, Shi Li, Yang Ding
Department of Entomology, China Agricultural University, Beijing 100193, China.
College of Forestry, Henan University of Science and Technology, Luoyang 471023, China.
Int J Mol Sci. 2017 Apr 14;18(4):773. doi: 10.3390/ijms18040773.
The superfamily Lauxanioidea is a significant dipteran clade including over 2500 known species in three families: Lauxaniidae, Celyphidae and Chamaemyiidae. We sequenced the first five (three complete and two partial) lauxanioid mitochondrial (mt) genomes, and used them to reconstruct the phylogeny of this group. The lauxanioid mt genomes are typical of the Diptera, containing all 37 genes usually present in bilaterian animals. A total of three conserved intergenic sequences have been reported across the Cyclorrhapha. The inferred secondary structure of 22 tRNAs suggested five substitution patterns among the Cyclorrhapha. The control region in the Lauxanioidea has apparently evolved very fast, but four conserved structural elements were detected in all three complete mt genome sequences. Phylogenetic relationships based on the mt genome data were inferred by Maximum Likelihood and Bayesian methods. The traditional relationships between families within the Lauxanioidea, (Chamaemyiidae + (Lauxaniidae + Celyphidae)), were corroborated; however, the higher-level relationships between cyclorrhaphan superfamilies are mostly poorly supported.
劳氏蝇总科是一个重要的双翅目分支，包括三个科（劳氏蝇科、瘦腹蝇科和扁眼蝇科）中的2500多种已知物种。我们对前五个（三个完整的和两个部分的）劳氏蝇类线粒体（mt）基因组进行了测序，并利用它们重建了该类群的系统发育。劳氏蝇类的线粒体基因组是双翅目的典型代表，包含两侧对称动物中通常存在的所有37个基因。在环裂亚目昆虫中总共报道了三个保守的基因间序列。推断出的22种tRNA的二级结构表明环裂亚目昆虫中有五种取代模式。劳氏蝇总科的控制区显然进化得非常快，但在所有三个完整的线粒体基因组序列中都检测到了四个保守的结构元件。基于线粒体基因组数据的系统发育关系通过最大似然法和贝叶斯法进行推断。劳氏蝇总科内各家族之间的传统关系（扁眼蝇科 + （劳氏蝇科 + 瘦腹蝇科））得到了证实；然而，环裂亚目超科之间的高级关系大多缺乏有力支持。
