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家蚕幼虫肠道微生物群因取食生菜叶而发生的变化。

Microbial shifts of the silkworm larval gut in response to lettuce leaf feeding.

作者信息

Liang Xue, Fu Yuming, Tong Ling, Liu Hong

机构信息

Laboratory of Environmental Biology and Life Support Technology, School of Biological Science and Medical Engineering, Beihang University, Beijing, China.

出版信息

Appl Microbiol Biotechnol. 2014 Apr;98(8):3769-76. doi: 10.1007/s00253-014-5532-y. Epub 2014 Feb 4.

Abstract

Silkworm (Bombyx mori L.) larvae were used as an ideal animal protein source for astronauts in the bioregenerative life support system (BLSS). Here, we compared the difference in bacterial communities of the silkworm larval gut between the BLSS rearing way (BRW) and the traditional rearing way (TRW) through culture-dependent approach, 16S rRNA gene analysis, and denaturing gradient gel electrophoresis (DGGE). The culture-dependent approach revealed that the numbers of gut bacteria of silkworm in the BRW significantly decreased compared with that of the TRW. The analysis of clone libraries showed that the gut microbiota in the BRW was significantly less diverse than that in the TRW. Acinetobacter and Bacteroides were dominant populations in the BRW, and Bacillus and Arcobacter dominated in the TRW. DGGE profiles confirmed the difference of silkworm gut bacterial community between two rearing ways. These results demonstrate that gut bacteria change from the BRW contributes to the decrease of silkworm physiological activity. This study increases our understanding of the change of silkworm gut microbiota in response to lettuce leaf feeding in the BRW. We could use the dominant populations to make probiotic products for nutrient absorption and disease prevention in the BLSS to improve gut microecology, as well as the yield and quality of animal protein.

摘要

家蚕(Bombyx mori L.)幼虫被用作生物再生生命支持系统(BLSS)中宇航员理想的动物蛋白来源。在此,我们通过纯培养法、16S rRNA基因分析和变性梯度凝胶电泳(DGGE),比较了BLSS饲养方式(BRW)和传统饲养方式(TRW)下家蚕幼虫肠道细菌群落的差异。纯培养法显示,与TRW相比,BRW饲养的家蚕肠道细菌数量显著减少。克隆文库分析表明,BRW的肠道微生物群多样性明显低于TRW。不动杆菌和拟杆菌是BRW中的优势种群,芽孢杆菌和弓形杆菌在TRW中占主导地位。DGGE图谱证实了两种饲养方式下家蚕肠道细菌群落的差异。这些结果表明,BRW导致的肠道细菌变化促使家蚕生理活性下降。本研究增进了我们对BRW中家蚕肠道微生物群因喂食生菜叶而发生变化的理解。我们可以利用优势种群制备益生菌产品,用于BLSS中的营养吸收和疾病预防,以改善肠道微生态,以及动物蛋白的产量和质量。

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