Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, China.
Analysis Centre of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou, China.
Sci Data. 2018 Dec 11;5:180285. doi: 10.1038/sdata.2018.285.
Lepidoptera (butterflies and moths) is a major insect order including important pollinators and agricultural pests, however their microbiomes are little studied. Here, using next-generation sequencing (NGS)-based shotgun metagenomics, we characterize both the biodiversity and functional potential of gut microbiota of a lepidopteran model insect, the silkworm Bombyx mori. Two metagenomes, including the standard inbred strain Dazao (P50) and an improved hybrid strain Qiufeng × Baiyu (QB) widely used in commercial silk production, were generated, containing 45,505,084 and 69,127,002 raw reads, respectively. Taxonomic analysis revealed that a total of 663 bacterial species were identified in P50 silkworms, while 322 unique species in QB silkworms. Notably, Enterobacter, Acinetobacter and Enterococcus were dominated in both strains. The further functional annotation was performed by both BlastP and MG-RAST against various databases including Nr, COG, KEGG, CAZy and SignalP, which revealed >5 × 10 protein-coding genes. These datasets not only provide first insights into all bacterial genes in silkworm guts, but also help to generate hypotheses for subsequently testing functional traits of gut microbiota in an important insect group.
鳞翅目（蝴蝶和蛾类）是一个主要的昆虫目，包括重要的传粉媒介和农业害虫，但它们的微生物组研究甚少。在这里，我们使用基于下一代测序（NGS）的 shotgun 宏基因组学，对鳞翅目模式昆虫家蚕的肠道微生物组的生物多样性和功能潜力进行了描述。生成了两个宏基因组，包括标准近交系 Dazao（P50）和广泛用于商业丝绸生产的改良杂交系 Qiufeng ⁇ × Baiyu（QB），分别包含 45,505,084 和 69,127,002 个原始读数。分类分析显示，P50 家蚕中共有 663 种细菌，而 QB 家蚕中有 322 种独特的细菌。值得注意的是，肠杆菌属、不动杆菌属和肠球菌属在家蚕的两个品系中都占据主导地位。进一步的功能注释是通过 BlastP 和 MG-RAST 对包括 Nr、COG、KEGG、CAZy 和 SignalP 在内的各种数据库进行的，共揭示了超过 5 × 10 个蛋白编码基因。这些数据集不仅提供了家蚕肠道中所有细菌基因的初步见解，还有助于提出假设，随后在家蚕这一重要昆虫群体中测试肠道微生物组的功能特征。
