Kurata K, Kurachi M, Hasegawa M, Kido H, Yamaguchi N
Department of Neuropsychiatry, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Jpn J Psychiatry Neurol. 1987 Jun;41(2):291-300. doi: 10.1111/j.1440-1819.1987.tb00415.x.
A simple high-performance liquid chromatographic method that allows the determination of 10 biogenic amines and related compounds for crude brain extracts has been developed. The compounds that can be quantified comprise norepinephrine, epinephrine, 3,4-dihydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylglycol, dopamine, 5-hydroxyindole-3-acetic acid, homovanillic acid, 3-methoxytyramine, 5-hydroxytryptamine and tryptophan. The detection system involves a three electrode-coulometric determination followed by fluorometric determination. This method is highly selective and sensitive, and in the present study, the usefulness of this methodology was confirmed by applying it to the determination of the levels of these various substances in 11 rat brain regions.
已开发出一种简单的高效液相色谱法，可用于测定粗脑提取物中的10种生物胺及相关化合物。可定量的化合物包括去甲肾上腺素、肾上腺素、3,4-二羟基苯乙酸、3-甲氧基-4-羟基苯乙二醇、多巴胺、5-羟基吲哚-3-乙酸、高香草酸、3-甲氧基酪胺、5-羟色胺和色氨酸。检测系统包括三电极库仑测定法，随后进行荧光测定。该方法具有高度的选择性和灵敏性，在本研究中，通过将该方法应用于测定11个大鼠脑区中这些不同物质的水平，证实了该方法的实用性。
