Borrebaeck Carl A K, Sturfelt Gunnar, Wingren Christer
Department of Immunotechnology and CREATE Health, Lund University, Lund, Sweden.
Methods Mol Biol. 2014;1134:67-78. doi: 10.1007/978-1-4939-0326-9_6.
Systemic lupus erythematosus (SLE) is a severe autoimmune connective tissue disease. Our current knowledge about the serum proteome, or serum biomarker panels, reflecting disease and disease status is still very limited. Affinity proteomics, represented by recombinant antibody arrays, is a novel, multiplex technology for high-throughput protein expression profiling of crude serum proteomes in a highly specific, sensitive, and miniaturized manner. The antibodies are deposited one by one in an ordered pattern, an array, onto a solid support. Next, the sample is added, and any specifically bound proteins are detected and quantified. The binding pattern is then converted into a relative protein expression map, or protein map, deciphering the composition of the sample at the molecular level. The methodology provides unique opportunities for delineating serum biomarkers reflecting SLE, thus paving the way for improved diagnosis, classification, and prognosis.
系统性红斑狼疮（SLE）是一种严重的自身免疫性结缔组织疾病。我们目前关于反映疾病及疾病状态的血清蛋白质组或血清生物标志物组合的知识仍然非常有限。以重组抗体阵列代表的亲和蛋白质组学是一种新颖的多重技术，能够以高度特异、灵敏和小型化的方式对粗血清蛋白质组进行高通量蛋白质表达谱分析。抗体以有序模式逐个沉积在固体支持物上形成阵列。接下来加入样品，检测并定量任何特异性结合的蛋白质。然后将结合模式转化为相对蛋白质表达图谱或蛋白质图谱，在分子水平上解读样品的组成。该方法为描绘反映SLE的血清生物标志物提供了独特机会，从而为改善诊断、分类和预后铺平了道路。
