Chen Ziqing, Dodig-Crnković Tea, Schwenk Jochen M, Tao Sheng-Ce
1Key Laboratory of Systems Biomedicine, (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240 China.
2Affinity Proteomics, SciLifeLab, KTH - Royal Institute of Technology, 171 65 Solna, Sweden.
Clin Proteomics. 2018 Feb 28;15:7. doi: 10.1186/s12014-018-9184-2. eCollection 2018.
The concept of antibody microarrays is one of the most versatile approaches within multiplexed immunoassay technologies. These types of arrays have increasingly become an attractive tool for the exploratory detection and study of protein abundance, function, pathways, and potential drug targets. Due to the properties of the antibody microarrays and their potential use in basic research and clinical analytics, various types of antibody microarrays have already been developed. In spite of the growing number of studies utilizing this technique, few reviews about antibody microarray technology have been presented to reflect the quality and future uses of the generated data. In this review, we provide a summary of the recent applications of antibody microarray techniques in basic biology and clinical studies, providing insights into the current trends and future of protein analysis.
抗体微阵列的概念是多重免疫分析技术中最具通用性的方法之一。这类阵列已日益成为用于蛋白质丰度、功能、信号通路及潜在药物靶点的探索性检测和研究的一种有吸引力的工具。由于抗体微阵列的特性及其在基础研究和临床分析中的潜在用途，已经开发出了各种类型的抗体微阵列。尽管利用该技术的研究数量不断增加，但很少有关于抗体微阵列技术的综述来反映所生成数据的质量和未来用途。在本综述中，我们总结了抗体微阵列技术在基础生物学和临床研究中的最新应用，深入探讨了蛋白质分析的当前趋势和未来发展。
