Conti Fabrizio, Alessandri Cristiano, Spinelli Francesca Romana, Capozzi Antonella, Martinelli Francesco, Recalchi Serena, Misasi Roberta, Valesini Guido, Sorice Maurizio
Dipartimento di Medicina Interna e Specialità Mediche, Sapienza Università di Roma, Policlinico Umberto I, Rome, Italy.
Methods Mol Biol. 2014;1134:95-101. doi: 10.1007/978-1-4939-0326-9_8.
Thin-layer chromatography (TLC) is a nonquantitative technique, which has been employed in the detection of antiphospholipid (aPL) antibodies. Antiphospholipid syndrome (APS) is the most frequently acquired thrombophilia, characterized by thrombosis and obstetric manifestations associated to an autoimmune trait, represented by the positivity of antiphospholipid (aPL) antibodies. Immunoassays for anticardiolipin (aCL) and anti-β2 glycoprotein I (aβ2GPI) antibodies and clotting tests for lupus anticoagulant (LA) represent the standard tests for the routine detection of aPL. The term "seronegative APS" has been used to describe patients with clinical manifestation of APS and persistently negative aPL assessed with routine assays. TLC immunostaining is a useful method for the detection of different antigenic targets of "antiphospholipid" antibodies; it is able to identify the reactivity of serum aPL experimented with purified phospholipid molecules with a different exposure compared to ELISA methods. This method seems to be applicable in patients who repeatedly tested negative for the standard aPL, i.e., aCL, aβ2GPI, and LA. Therefore, this technique may be proposed as a second step test for the diagnosis of APS.
薄层色谱法（TLC）是一种非定量技术，已用于抗磷脂（aPL）抗体的检测。抗磷脂综合征（APS）是最常见的获得性血栓形成倾向，其特征为与自身免疫特征相关的血栓形成和产科表现，以抗磷脂（aPL）抗体阳性为代表。抗心磷脂（aCL）和抗β2糖蛋白I（aβ2GPI）抗体的免疫测定以及狼疮抗凝物（LA）的凝血试验是aPL常规检测的标准试验。“血清阴性APS”一词已用于描述具有APS临床表现且通过常规检测aPL持续呈阴性的患者。TLC免疫染色是检测“抗磷脂”抗体不同抗原靶点的有用方法；与酶联免疫吸附测定（ELISA）方法相比，它能够识别血清aPL与不同暴露的纯化磷脂分子反应的情况。该方法似乎适用于标准aPL（即aCL、aβ2GPI和LA）反复检测呈阴性的患者。因此，该技术可作为APS诊断的第二步检测方法。
