Gnarpe H, Unge P, Blomqvist C, Mäkitalo S
Department of Clinical Bacteriology, Gävle Central Hospital, Sweden.
APMIS. 1988 Feb;96(2):128-32.
Campylobacter pylori was isolated more often from patient with peptic ulcers and in an age-related manner in a material of 395 consecutive patients referred for gastroscopy. Direct microscopy was done with Gram and Acridine Orange stain and found too insensitive for practical use. All patients were investigated serologically with an enzyme immunoassay which showed excellent correlation with positive cultures for C. pylori. The findings were discussed, and the enzyme immunoassay, with a negative predictive value of 0.99, was found to be a valuable tool for primary screening of patients suspected of being carriers of C. pylori.
在395例因胃镜检查转诊的连续患者样本中，幽门螺杆菌在消化性溃疡患者中分离率更高，且与年龄有关。采用革兰氏染色和吖啶橙染色进行直接显微镜检查，发现其实际应用时敏感性不足。所有患者均采用酶免疫测定法进行血清学检测，结果显示该方法与幽门螺杆菌阳性培养结果具有良好的相关性。对研究结果进行了讨论，发现酶免疫测定法的阴性预测值为0.99，是对疑似幽门螺杆菌携带者进行初步筛查的有价值工具。
