Wang Xin, Fan Yahan, Shi Ronghua, Li Jing, Zhao Shuming
Department of Blood Transfusion, Southwest Hospital, the Third Military Medical University , Chongqing , P.R. China.
Platelets. 2015;26(1):72-9. doi: 10.3109/09537104.2013.872772. Epub 2014 Feb 5.
Trehalose is widely used as a cryoprotective reagent to preserve various cells. Platelet additive solution-III (PAS) has been used to maintain platelet function, benefit the virus inactivation, and extend the storage period. PAS with trehalose (PAS-III M + T) may effectively protect platelets (PLTs) at a relatively low temperature (10 °C). The apheresis PLTs from six donors were divided into two groups. Group A was stored in PAS-III M + T at 10 °C as experimental group and group B in plasma at 22 °C as control group. The samples were collected on different storage dates, and multiple parameters were determined or investigated for in vitro studies. The in vivo recovery and survival of rabbit PLTs stored in the same conditions, and then labeled with (51)Cr were measured and evaluated using a rabbit model of thrombocytopenia. Over 9 days, P-selectin expression increased significantly in a time-dependent manner in both groups (n = 6). The levels of the hypotonic shock reaction and PLT aggregation rate decreased in both groups and were significantly higher in group A than B after 1 day of storage. The lactate dehydrogenase (LDH) release and glucose (GLU) consumption increased similarly, but the levels were significantly lower in group A than B. The pH decreased significantly after 5 days of storage in group B but did not change in group A. After 5 days, the morphology of the PLTs in group B maintained a more normal shape than that of group A. The recovery and survival of PLTs stored in both groups were not significantly different (p > 0.05). The bacteria growth was not examined out in both groups for up to 5 (group A) and 9 (group B) days. Storage of PLTs in the modified PAS at low temperature was more effective in protecting PLT functions than that of standard storage method and may have the potential to decrease the risk of PLT activation and bacterial contamination.
海藻糖被广泛用作一种冷冻保护剂来保存各种细胞。血小板添加剂溶液III（PAS）已被用于维持血小板功能、有利于病毒灭活并延长储存期。含有海藻糖的PAS（PAS-III M+T）可能在相对低温（10°C）下有效保护血小板（PLT）。将来自6名供体的单采血小板分为两组。A组作为实验组在10°C下储存在PAS-III M+T中，B组作为对照组在22°C下储存在血浆中。在不同储存日期采集样本，并测定或研究多个参数以进行体外研究。使用血小板减少症兔模型测量并评估在相同条件下储存然后用（51）Cr标记的兔PLT的体内回收率和存活率。在9天内，两组（n=6）中P-选择素表达均以时间依赖性方式显著增加。两组的低渗休克反应水平和PLT聚集率均下降，且储存1天后A组显著高于B组。乳酸脱氢酶（LDH）释放和葡萄糖（GLU）消耗同样增加，但A组水平显著低于B组。储存5天后B组pH显著下降而A组未变化。5天后，B组PLT的形态比A组保持更正常的形状。两组储存的PLT的回收率和存活率无显著差异（p>0.05）。在长达5天（A组）和9天（B组）内两组均未检测到细菌生长。将PLT低温储存在改良的PAS中比标准储存方法更有效地保护PLT功能，并且可能有降低PLT激活和细菌污染风险的潜力。
