Department of General, Visceral and Transplant Surgery, University Hospital of the RWTH Aachen, Pauwelsstraße 30, 52074, Aachen, Germany,
Hernia. 2014 Aug;18(4):571-8. doi: 10.1007/s10029-014-1220-1. Epub 2014 Feb 6.
As part of the foreign body reaction, mesh filaments are surrounded by an infiltrate of inflammatory cells. Though macrophages are considered as being predominant, little is known about the origin of other cells.
On 55 meshes explanted from humans, we characterised the cells in the inflammatory infiltrate of the granuloma by immunohistochemistry using 10 cellular markers: CD3+ lymphocytes, CD4+ T helper cells, CD8+ cytotoxic T cells, CD20+ B lymphocytes, CD34+ stem cells, CD45R0+ leucocytes, CD68+ macrophages, Mib1 for proliferation, Vimentin for mesenchymal origin, and Desmin for myocytes. Collagen deposits were analysed after staining with Sirius Red.
More than 80 % of the cells in the infiltrate showed a positive expression of CD68, CD8, CD45R0 and Vimentin. CD4 and Desmin were seen in 30-80 % of the cells, unaffected by material or time. A score summarising the expression of all markers positively correlated significantly with an increased percentage of collagen type III (green) in the mesh wound. The analysis of collagen deposits was only affected to a small degree by size of area for investigation.
At the vicinity of the mesh filaments, the accumulated inflammatory cells represent a mixture of cells of various origins. The high expression of at least four markers requires co-expression of different surface markers and thus confirms the existence of multiple transition forms instead of dominance of just macrophages. This offers new options for interventions to attenuate the inflammatory reaction of mesh implants.
作为异物反应的一部分，网丝被炎症细胞浸润所包围。尽管巨噬细胞被认为是主要的，但其他细胞的来源知之甚少。
我们对从人类体内取出的 55 个网片进行研究，通过免疫组织化学方法使用 10 种细胞标志物（CD3+淋巴细胞、CD4+辅助性 T 细胞、CD8+细胞毒性 T 细胞、CD20+B 淋巴细胞、CD34+干细胞、CD45R0+白细胞、CD68+巨噬细胞、Mib1 用于增殖、Vimentin 用于间充质起源、Desmin 用于肌细胞）来描述肉芽肿炎症浸润中的细胞特征。用天狼星红染色后分析胶原沉积。
浸润中的细胞超过 80%表达 CD68、CD8、CD45R0 和 Vimentin。CD4 和 Desmin 在 30-80%的细胞中表达，不受材料或时间的影响。总结所有标记物阳性表达的评分与网片伤口中 III 型胶原（绿色）的百分比增加显著相关。胶原沉积的分析仅受到研究面积大小的微小影响。
在网丝附近，积聚的炎症细胞代表了各种来源的细胞混合物。至少四种标志物的高表达需要不同表面标志物的共表达，从而证实了多种过渡形式的存在，而不是仅仅巨噬细胞的主导地位。这为减轻网片植入物炎症反应的干预措施提供了新的选择。
