Soft Tissue Biomechanics and Tissue Engineering, Department of Biomedical Engineering, and ‡Institute for Complex Molecular Systems, Eindhoven University of Technology , P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
Biomacromolecules. 2014 Mar 10;15(3):821-9. doi: 10.1021/bm4016418. Epub 2014 Feb 13.
Electrospun scaffolds for in situ tissue engineering can be prepared with different fiber diameters to influence cell recruitment, adhesion, and differentiation. For cardiovascular applications, we investigated the impact of different fiber diameters (2, 5, 8, and 11 μm) in electrospun poly(ε-caprolactone) scaffolds on endothelial colony forming cells (ECFCs) in comparison to mature endothelial cells (HUVECs). In 2D cultures and on 2 μm fiber scaffolds, ECFC morphology and phenotype resemble those of HUVECs. When cultured on scaffolds with 5-11 μm fibers, a different behavior was detected. HUVECs developed a cytoskeleton organized circumferentially around the fibers, with collagen alignment in the same direction. ECFCs, instead, aligned the cytoskeleton along the scaffold fiber axis and deposited a homogeneous layer of collagen over the fibers; moreover, a subpopulation of ECFCs gained the αSMA marker. These results showed that ECFCs do not behave like mature endothelial cells in a 3D fibrous environment.
用于原位组织工程的静电纺丝支架可以用不同的纤维直径来影响细胞的募集、黏附和分化。对于心血管应用，我们研究了不同纤维直径（2、5、8 和 11 μm）的静电纺丝聚（ε-己内酯）支架对内皮集落形成细胞（ECFCs）的影响，与成熟的内皮细胞（HUVECs）进行了比较。在 2D 培养物和 2 μm 纤维支架上，ECFC 的形态和表型与 HUVECs 相似。当在 5-11 μm 纤维支架上培养时，检测到不同的行为。HUVECs 围绕纤维形成了一个呈圆周排列的细胞骨架，胶原纤维也沿同一方向排列。相反，ECFCs 将细胞骨架沿着支架纤维轴排列，并在纤维上沉积一层均匀的胶原；此外，ECFCs 的一个亚群获得了αSMA 标志物。这些结果表明，ECFCs 在 3D 纤维环境中不像成熟的内皮细胞那样起作用。
