Vinodhini A, Govindaraju K, Singaravelu G, Sadiq A Mohamed, Kumar V Ganesh
Nanoscience Division, Department of Zoology, Thiruvalluvar University, Vellore 632 004, India.
Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
Colloids Surf B Biointerfaces. 2014 May 1;117:480-6. doi: 10.1016/j.colsurfb.2014.01.006. Epub 2014 Jan 15.
In the present investigation, the cardioprotective nature of proanthocyanidin (PAC)-synthesized gold nanoparticles (AuNPs) is addressed in detail. There was a rapid reduction of gold metal ions by PAC and the new-genre AuNPs exhibited remarkable in vitro stability both in biological and chemical solutions. Transmission electron microscopy (TEM) indicated that the newly formed nanoparticles ranged in size from 17 to 29 nm (∼24 nm). MTT assay carried out with peripheral blood mononuclear cells demonstrated the non-toxic nature of AuNPs. PAC-synthesized AuNPs showed cardioprotective action in isoproterenol-induced myocardial injury at a lowest dosage (9 mg kg(-1)). Cardiac marker enzymes and antioxidant parameters in serum and heart tissue were also measured.
在本研究中，详细探讨了原花青素（PAC）合成的金纳米颗粒（AuNPs）的心脏保护特性。PAC能快速还原金金属离子，新型AuNPs在生物和化学溶液中均表现出显著的体外稳定性。透射电子显微镜（TEM）显示新形成的纳米颗粒尺寸范围为17至29纳米（约24纳米）。用外周血单核细胞进行的MTT试验证明了AuNPs的无毒性质。PAC合成的AuNPs在最低剂量（9毫克/千克(-1)）时对异丙肾上腺素诱导的心肌损伤显示出心脏保护作用。还测量了血清和心脏组织中的心脏标志物酶和抗氧化参数。
