Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Department of Safety and Quality of Fruits and Vegetables, Haid-und-Neu-str. 09, D-76131 Karlsruhe, Germany.
Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Department of Safety and Quality of Fruits and Vegetables, Haid-und-Neu-str. 09, D-76131 Karlsruhe, Germany.
Int J Food Microbiol. 2014 Apr 3;175:20-9. doi: 10.1016/j.ijfoodmicro.2014.01.010. Epub 2014 Jan 25.
In this study the differentially expressed protein population of Penicillium verrucosum grown either in the dark or under light with a wavelength of 450nm has been analyzed. Light of short wavelength led to oxidative stress in the fungal cell; under this condition the mycotoxin biosynthesis revealed a mutual shift from ochratoxin A to citrinin. Using a proteomic approach combining an optimized protein extraction method with 2-dimensional SDS-PAGE followed by HPLC-ESI-TOF-MS/MS mass spectrometric analysis, initially 56 significantly differential proteins (light vs. dark) were detected comprising proteins of a broad range of isoelectric points and molecular masses. In total, 46 proteins could be identified further by database query, most of these proteins are assumed to be involved in response to stress (e.g. antioxidative proteins, heat shock proteins) and general metabolic processes (e.g. glycolysis, ATP supply). Proteome analyses are necessary to unravel the regulation of secondary metabolite biosynthesis at a translational level. This may enable identification of proteins which are involved in mycotoxin biosynthesis, adaption processes or even stress compensation mechanisms. This study depicts the first proteome analysis of P. verrucosum.
在这项研究中，分析了在黑暗或波长为 450nm 的光下生长的产黄青霉的差异表达蛋白群体。短波长的光会导致真菌细胞中的氧化应激；在这种条件下，霉菌毒素生物合成显示出从桔青霉素到桔青霉素的相互转移。使用一种蛋白质组学方法，结合优化的蛋白质提取方法与 2 维 SDS-PAGE 后 HPLC-ESI-TOF-MS/MS 质谱分析，最初检测到 56 种明显差异的蛋白质（光与暗），这些蛋白质包含广泛的等电点和分子量范围的蛋白质。总共，通过数据库查询可以进一步鉴定出 46 种蛋白质，其中大多数蛋白质被认为参与应激反应（如抗氧化蛋白、热休克蛋白）和一般代谢过程（如糖酵解、ATP 供应）。蛋白质组学分析对于揭示次生代谢物生物合成在翻译水平上的调控是必要的。这可能有助于鉴定参与霉菌毒素生物合成、适应过程甚至应激补偿机制的蛋白质。本研究描述了产黄青霉的首次蛋白质组学分析。
