Nöbauer Katharina, Hummel Karin, Mayrhofer Corina, Ahrens Maike, Setyabudi Francis M C, Schmidt-Heydt Markus, Eisenacher Martin, Razzazi-Fazeli Ebrahim
VetCore Facility for Research, University of Veterinary Medicine, Vienna, Austria.
Department for Biomedical Sciences, Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Vienna, Austria.
Proteomics. 2017 May;17(9). doi: 10.1002/pmic.201600467.
Mass spectrometric identification of proteins in species lacking validated sequence information is a major problem in veterinary science. In the present study, we used ochratoxin A producing Penicillium verrucosum to identify and quantitatively analyze proteins of an organism with yet no protein information available. The work presented here aimed to provide a comprehensive protein identification of P. verrucosum using shotgun proteomics. We were able to identify 3631 proteins in an "ab initio" translated database from DNA sequences of P. verrucosum. Additionally, a sequential window acquisition of all theoretical fragment-ion spectra analysis was done to find differentially regulated proteins at two different time points of the growth curve. We compared the proteins at the beginning (day 3) and at the end of the log phase (day 12).
在缺乏经过验证的序列信息的物种中进行蛋白质的质谱鉴定是兽医学中的一个主要问题。在本研究中，我们使用产赭曲霉毒素A的疣孢青霉来鉴定和定量分析一种尚无蛋白质信息的生物体的蛋白质。此处展示的工作旨在利用鸟枪法蛋白质组学对疣孢青霉进行全面的蛋白质鉴定。我们能够在一个根据疣孢青霉DNA序列进行“从头”翻译的数据库中鉴定出3631种蛋白质。此外，还进行了所有理论碎片离子谱分析的序列窗口数据采集，以找出在生长曲线的两个不同时间点差异调节的蛋白质。我们比较了对数期开始时（第3天）和结束时（第12天）的蛋白质。
