Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Invest Ophthalmol Vis Sci. 2014 Feb 24;55(2):1118-23. doi: 10.1167/iovs.13-13523.
To define the phenotype and location of antigen-presenting cells (APCs) in normal donor human corneas, and to assess the response of APC to herpes simplex virus type 1 (HSV-1) infection.
Donor human corneal tissue was analyzed by fluorescence confocal microscopy and flow cytometry to determine the phenotype and location of tissue-resident APCs. Confocal fluorescence microscopy was also utilized to investigate the response of corneal resident APCs to ex vivo infection with HSV-1.
CD11c(+) dendritic cells (DCs) and CD207(+) Langerhans cells (LCs) were situated predominantly in the basal epithelium and CD68(+) macrophages in the anterior stroma of human corneas. The majority of DCs expressed major histocompatibility complex class II. Corneal resident APCs colocalized with HSV-1-infected corneal cells within 8 to 16 hours of ex vivo infection.
The stratification of APCs found in human corneas is very similar to that previously reported in mice, confirming the relevance of murine models for the study of corneal APCs. Furthermore, corneal resident APCs are capable of rapidly mobilizing to the site of trauma and HSV-1 infection within the cornea.
定义正常供体人角膜中抗原呈递细胞 (APC) 的表型和位置，并评估 APC 对单纯疱疹病毒 1 型 (HSV-1) 感染的反应。
通过荧光共聚焦显微镜和流式细胞术分析供体人角膜组织，以确定组织驻留 APC 的表型和位置。还利用共聚焦荧光显微镜研究角膜常驻 APC 对 HSV-1 体外感染的反应。
CD11c(+)树突状细胞 (DC) 和 CD207(+)朗格汉斯细胞 (LC) 主要位于基底层上皮，而 CD68(+)巨噬细胞位于人前基质中。大多数 DC 表达主要组织相容性复合物 II 类。在体外感染后 8 至 16 小时，角膜常驻 APC 与 HSV-1 感染的角膜细胞共定位。
在人角膜中发现的 APC 分层与先前在小鼠中报道的分层非常相似，证实了小鼠模型在研究角膜 APC 方面的相关性。此外，角膜常驻 APC 能够迅速迁移到角膜内创伤和 HSV-1 感染的部位。
