Sahpazidou Despina, Geromichalos George D, Stagos Dimitrios, Apostolou Anna, Haroutounian Serkos A, Tsatsakis Aristidis M, Tzanakakis George N, Hayes A Wallace, Kouretas Dimitrios
Cell Culture, Molecular Modelling and Drug Design Lab., Symeonidion Research Center, Theagenion Cancer Hospital, Thessaloniki 54639, Greece.
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou, Larissa 41221, Greece.
Toxicol Lett. 2014 Oct 15;230(2):218-24. doi: 10.1016/j.toxlet.2014.01.042. Epub 2014 Feb 6.
A major part of the wineries' wastes is composed of grape stems which are discarded mainly in open fields and cause environmental problems due mainly to their high polyphenolic content. The grape stem extracts' use as a source of high added value polyphenols presents great interest because this combines a profitable venture with environmental protection close to wine-producing zones. In the present study, at first, the Total Polyphenolic Content (TPC) and the polyphenolic composition of grape stem extracts from four different Greek Vitis vinifera varieties were determined by HPLC methods. Afterwards, the grape stem extracts were examined for their ability to inhibit growth of colon (HT29), breast (MCF-7 and MDA-MB-23), renal (786-0 and Caki-1) and thyroid (K1) cancer cells. The cancer cells were exposed to the extracts for 72 h and the effects on cell growth were evaluated using the SRB assay. The results indicated that all extracts inhibited cell proliferation, with IC₅₀ values of 121-230 μg/ml (MCF-7), 121-184 μg/ml (MDA-MD-23), 175-309 μg/ml (HT29), 159-314 μg/ml (K1), 180-225 μg/ml (786-0) and 134->400 μg/ml (Caki-1). This is the first study presenting the inhibitory activity of grape stem extracts against growth of colon, breast, renal and thyroid cancer cells.
酒庄废弃物的主要部分由葡萄茎组成，这些葡萄茎主要被丢弃在露天场地，主要因其高多酚含量而导致环境问题。葡萄茎提取物作为高附加值多酚的来源具有很大的吸引力，因为这将一项有利可图的业务与葡萄酒产区附近的环境保护结合起来。在本研究中，首先，通过高效液相色谱法测定了来自四个不同希腊葡萄品种的葡萄茎提取物的总多酚含量（TPC）和多酚组成。之后，检测了葡萄茎提取物抑制结肠（HT29）、乳腺（MCF-7和MDA-MB-23）、肾（786-0和Caki-1）和甲状腺（K1）癌细胞生长的能力。将癌细胞暴露于提取物中72小时，并使用SRB试验评估对细胞生长的影响。结果表明，所有提取物均抑制细胞增殖，IC₅₀值分别为121 - 230μg/ml（MCF-7）、121 - 184μg/ml（MDA-MD-·23）、175 - 309μg/ml（HT29）、159 - 314μg/ml（K1）、180 - 225μg/ml（786-0）和134 -> 400μg/ml（Caki-1）。这是第一项展示葡萄茎提取物对结肠、乳腺、肾和甲状腺癌细胞生长具有抑制活性的研究。
