Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Int J Mol Sci. 2022 Nov 28;23(23):14873. doi: 10.3390/ijms232314873.
Phenolic extracts from berry seeds have been extensively studied for their health benefits. However, few studies have been conducted on the effects of phenolic extracts from L. canes and berry stems. The Chinese spine grape ( Foex) is an important and widely distributed wild species of L. The present study explored the metabolomic profile and evaluated the antioxidant activity of phenolic compounds in extracts from Foex. canes and stems, with a focus on their role in preventing DNA damage caused by free radicals and inhibiting the growth of breast (MCF-7) and cervical (HeLa) cancer cells. Total phenolic compounds in the dried berry stems of spine grapes were higher than that in vine canes. Analysis of the extracts showed that proanthocyanins, epicatechin, catechin, and phenolic acid were the main phenolic compounds in Foex, but in higher quantities in berry stems than in vine canes. However, -resveratrol and kaempferol 3--glucoside were present in the vine canes but not in the berry stems. Antioxidant analysis by FRAP and ABTS showed that extracts from berry stems and vine canes had a higher antioxidant activity than thinned young fruit shoots before flowering, leaves, peel, pulp, and seeds in Foex. Moreover, the antioxidant activity of extracts from berry stems was higher than that in other grape species, except for muscadine. In vitro analyses further showed that the extracts significantly increased HO scavenging ability and conferred a protective effect against DNA damage. Furthermore, a low concentration of phenolic compounds in extracts from the vine canes and berry stems of spine grapes inhibited the proliferation of the MCF-7 and Hela cancer cells. These research results provided some important useful information for the exploitation of Foex canes and berry stems and indicated that canes and stems of Foex had good antioxidant properties, anticancer activity and prevented DNA damage, providing evidence for medical utilization of Foex.
浆果种子的酚类提取物因其对健康的益处而被广泛研究。然而，对中华猕猴桃（ Foex）藤和浆果茎的酚类提取物的影响研究较少。中华猕猴桃是猕猴桃属的一个重要且广泛分布的野生种。本研究探索了 Foex 藤和茎提取物的代谢组学特征，并评估了其酚类化合物的抗氧化活性，重点研究了其预防自由基引起的 DNA 损伤和抑制乳腺癌（MCF-7）和宫颈癌（HeLa）细胞生长的作用。中华猕猴桃干浆果茎中的总酚类化合物高于藤。提取物分析表明，原花青素、表儿茶素、儿茶素和酚酸是 Foex 的主要酚类化合物，但在浆果茎中的含量高于藤。然而，-白藜芦醇和山奈酚 3--葡萄糖苷存在于藤中，而不存在于浆果茎中。FRAP 和 ABTS 抗氧化分析表明，与 Foex 的幼果嫩枝（开花前）、叶片、果皮、果肉和种子相比，藤和浆果茎提取物具有更高的抗氧化活性。此外，藤和浆果茎提取物的抗氧化活性高于其他葡萄品种，除麝香葡萄外。体外分析进一步表明，提取物显著增加了 HO 清除能力，并对 DNA 损伤提供了保护作用。此外，藤和浆果茎的提取物中的低浓度酚类化合物抑制了 MCF-7 和 Hela 癌细胞的增殖。这些研究结果为中华猕猴桃藤和浆果茎的开发提供了一些重要的有用信息，并表明中华猕猴桃藤和浆果茎具有良好的抗氧化、抗癌活性和预防 DNA 损伤的特性，为中华猕猴桃的药用利用提供了证据。
