苜蓿花叶病毒RNA 3前导序列中的可变重复序列和聚（A）序列

Variable repeats and poly (A)-stretches in the leader sequence of alfalfa mosaic virus RNA 3.

作者信息

Langereis K, Mugnier M A, Cornelissen B J, Pinck L, Bol J F

机构信息

Department of Biochemistry, State University of Leiden, P.O. Box 9505, 2300 RA Leiden, The Netherlands.

出版信息

Virology. 1986 Oct 30;154(2):409-14. doi: 10.1016/0042-6822(86)90468-x.

DOI:10.1016/0042-6822(86)90468-x

PMID:18640652

Abstract

The complete nucleotide sequence of RNA 3 of the L strain of alfalfa mosaic virus (AlMV) was deduced and the 5'-terminal sequence of RNA 3 of the S-strain was revised. A comparison of the RNA sequences of AlMV strains L, S, and M showed that a sequence of 27 to 30 nucleotides is repeated two times in the 5' noncoding regions of all strains. In addition, sequences of 56 and 75 nucleotides are duplicated in the leaders of strain S and L, respectively. An A-rich sequence of 10 nucleotides, present in strain S and M, was found to be extended to 40 nucleotides in strain L. The data provide further information on the fidelity of RNA-dependent RNA polymerases.

摘要

推导了苜蓿花叶病毒（AlMV）L株系RNA 3的完整核苷酸序列，并对S株系RNA 3的5'末端序列进行了修订。对AlMV L、S和M株系的RNA序列比较表明，所有株系的5'非编码区均有一段27至30个核苷酸的序列重复两次。此外，S株系和L株系的前导序列中分别重复了56和75个核苷酸的序列。在S株系和M株系中存在的一段富含10个核苷酸的A序列，在L株系中延伸至40个核苷酸。这些数据为RNA依赖性RNA聚合酶的保真度提供了更多信息。